Matsushita Electric Industrial Co., Ltd.

Panasonic Corporation

Other

Kobe University

The Ritsumeikan Trust

Matsushita Technical Information Services Co., Ltd.

Hirokazu Sugihara

Hiroaki Oka

Makoto Taketani

Nobuhiko Ozaki

Akihito Kamei

Tadayasu Mitsumata

Ryuta Ogawa

Yasushi Kobayashi

Hiroshi Iwasaki

Tetsuo Yukimasa

Hidenobu Yaku

Ken Shimono

Gary S Lynch

Satoshi Konishi

Shuhei Tanaka

Katsuyuki Tsuji

Atsuo Tamura

Yukifumi Yoshimoto

Kentaro Onizuka

The present invention provides an apparatus for measuring activity signals of a biological sample comprising: a measurement chamber (A) storing a target liquid containing a biological sample; a porous insulating substrate () provided with a measurement electrode () on at least one side; and a conveying device () which conveys the target liquid stored in the measurement chamber (A) and passes the target liquid through the porous insulating substrate () from the measurement electrode () side, wherein the conveying device () is operated to trap the biological sample contained in the target liquid onto the measurement electrode (), so that the activity signals of the biological sample are measured through the measurement electrode (). According to the apparatus, activity signals emitted from the biological sample can be detected easily, rapidly and accurately.

Apparatus and method for measuring activity signals of biological samples

The present invention provides a chipthat enables an immunoassay with high accuracy and without requiring a high volatile reagent. The chipincludes a chip substrate, a reagent mixtureand potassium ferricyanidethat are immobilized leaving a space in between on the chip substrate. The reagent mixtureincludes at least one selected from NADP and NADPH, malate dehydrogenase, and a substrate of malate dehydrogenase. The chipfurther includes diaphorase, immobilized on the chip substrate, as a part of or a component other than the reagent mixture

Chip for electrochemical immunoassay

Immobilization of malate dehydrogenase on a substrate using a glycerol solution containing malate dehydrogenase is achieved through dropping a mixed solution obtained by adding at least one selected from malic acid and malate to the glycerol containing malate dehydrogenase on the substrate, and drying it thereon. It is preferable to prepare the mixed solution by adding the malate to the glycerol solution containing malate dehydrogenase. The malate is preferably at least one selected from potassium malate and sodium malate.

Method of immobilizing malate dehydrogenase on a substrate

A measurement deviceincludes a measurement cell. The measurement cellincludes a substratesand a substrateformed on the substrate. The substratesandare bonded to each other. Thus, a flow passis formed in the measurement cell. As shown in FIG., on the substrateof the measurement cell, provided are a concave well, a protruding microelectrodeconnected to a terminaland formed on a side wall surface of the well, communicating tubesandconnected to both of side surfaces of the well(i.e., the flow pass) to face to each other, resistance bathsandconnected to the communicating tubesand, thin film resistorsandarranged in the resistance bathsand, and a reference electrodeconnected to a terminal

Measurement device and method for measuring electric signal from biological sample

A peptide nanofiber having conductivity is provided. A conductive peptide nanofiber which includes a nanofiber formed through a manner of self-assembly of a peptide that has a nanofiber-forming ability and consists of an amino acid sequence of Xaa-Phe-Ile-Val-Ile-Phe-Xaa (SEQ ID NO: 1, wherein N-terminal Xaa is an arbitrary amino acid residue Xaa; C-terminal Xaa is an arbitrary amino acid residue Xaa; and Xaaand Xaaare an amino acid having an acidic side chain, an amino acid having a basic side chain, or an amino acid having a side chain with polarity according as acidity and basicity) or a derivative of the peptide and a conductive substance added thereto, the aforementioned conductive substance being added to an amino group of the peptide or the derivative.

Conductive peptide nanofiber and method of manufacture of the same

A cell potential measurement apparatus, which uses a planar electrode enabling a multi-point simultaneous measurement of potential change arising from cell activities, is provided which can conduct measurements accurately and efficiently as well as can improve convenience of arranging measurement results. According to the configuration of the cell potential measurement apparatus of this invention, it includes an integrated cell holding instrument, which includes a planar electrode provided with a plurality of microelectrodes arranged in a matrix form on the surface of a substrate, a cell holding part for placing cells thereon, drawer patterns from the microelectrodes, and electric contact points for outside connections; an optical observation means for optical observations of cells; a stimulation signal supply means to be connected to the cell holding instrument for providing electric stimulation to the cells; and a signal processing means to be connected to the cell holding instrument for processing an output signal arising from electric physiological activities of the cells. It is preferable that a cell culturing means is also provided for maintaining a culture atmosphere of the cells placed on the integrated cell holding instrument.

Cell potential measurement apparatus having a plurality of microelectrodes

A device for detecting a physicochemical change in a biological sample comprising at least one measuring electrode provided by causing a conductive material to enter into a porous film, and a conductor connected to the measuring electrode.

Method and apparatus for detecting physicochemical changes emitted by biological sample

A multiple electrode includes a plurality of micro-electrodes provided on a substrate, and a wiring portion for providing an electrical signal to the micro-electrodes or extracting an electrical signal from the micro-electrodes. Each micro-electrode has porous conductive material on its surface, and the impedance of the micro-electrode is 50 k&#x3a9; or less. Preferably, the porous conductive material is gold, and formed by the passage of current at a current density of 1.0 to 5.0 A/dmfor 10 to 360 sec. The multiple electrode may include micro-electrodes provided on a substrate in the form of a matrix, a lead line connected to the micro-electrodes, and an electrical junction connected to an end of the lead line.

Extracellular recording electrode

A sensor capable of simply and quickly measuring the electrical activities of a number of cells simultaneously and a device comprising the sensor and capable of detecting the signal transmission between subject cells are provided. The sensor comprises a plurality of electrodes provided on a substrate. The electrodes individually measure a signal generated by a biological sample held by each of the electrodes. Each of the electrodes comprises a depression for holding the biological sample, a throughhole which is in communication with the depression and penetrates to a rear side of the substrate, an electrode portion, and a drawing line from the electrode portion. The electrodes are arranged so that the samples held by the depressions electrically communicate with one another.

Signal detecting sensor provided with multi-electrode

A method for extracting a signal of interest from a signal generated from at least one subject, comprises the steps of (A) obtaining a time-series signal generated from the subject as a data group consisting of a plurality of pieces of data, (B) obtaining a plurality of extracted data groups from the data group, wherein each extracted data group comprises a predetermined number of pieces of data selected from the data group, (C) calculating standard deviations of the plurality of extracted data groups to obtain a standard deviation group, and (D) referencing each standard deviation included in the standard deviation group and selecting the signal of interest.

Method for extracting significant signal, recording medium and program

A multiple electrode for measuring electro-physiological characteristics of a biological specimen includes a plurality of micro-electrode provided on a first region on a substrate, and a reference electrode provided in a second region on the substrate. The reference electrode includes at least one stimulus reference electrode for applying an electrical signal to the plurality of micro-electrodes. Preferably, the reference electrode includes at least one measurement reference electrode for detecting an electrical signal from the plurality of micro-electrodes, and the stimulus reference electrode is electrically insulated from the measurement reference electrode. Preferably, the second region is placed at a distance from an outer edge of the first region, and surrounds the first region.

Extracellular recording multiple electrode

A method of measuring the physical and chemical properties of tissue or cells and a device for the same is provided, with which the physical and chemical environment of the tissue or cells can be changed arbitrarily corresponding to experimental necessities. The device comprises a systemfor keeping the physical and chemical environment surrounding the biological tissue or cells constant, a systemfor arbitrarily changing the physical and chemical environment, observation systemsandfor observing the physical and chemical properties of the tissue or cells, and a systemfor comparing the change of the physical and chemical properties of the tissue or cells before and after changing the physical and chemical environment. The observation systemis a potential measurement device for measuring the electrophysiological properties of the tissue or cells. This device comprises an integrated cell placement deviceprovided with a plurality of microelectrodeson a substrate, a cell placement portionfor placing the tissue or cells on the microelectrodes, and a wiring patternfor applying an electrical signal to the microelectrodesand extracting an electrical signal from the microelectrodes

Method for measuring physiocochemical properties of tissues of cells, method for examining chemicals, and apparatus therefor

This relates to methods for the detection of psychoactive compounds in an in vitro neuronal tissue sample by detecting oscillations of extracellular voltage desirably before and after the introduction of a candidate sample onto an in vitro neuronal tissue sample and for devices useful in practicing the methods. Analysis of the extracellular voltage parameters leads to indication of the presence of psychoactive material in the candidate sample and information as to its pharmacological activity and/or composition. Further, it relates to a process of initiating and maintaining the presence of repetitive neuronal activity within the in vitro sample. Additionally, this includes a method for the stimulation of or initiation of repetitive neuronal activity, e.g., EEG, in an in vitro neuronal tissue sample by introducing a stimulating composition comprising compounds that facilitate or mimic the actions of acetylcholine, serotonin, or catecholamines, such as carbachol, or by subjecting the in vitro neuronal sample to electrical stimulation parameters.

Methods and device for in vitro detection and characterization of psychoactives using analysis of repetitive electrical activity in a neuronal sample

A method of observing a physical and chemical property of a tissue or cell by using an apparatus which comprises at least a cell culturing means, an environment conditioning means, an observing means and a comparing means, comprising the steps of (A) culturing the tissue or cell by the cell culturing means, (B) maintaining a first physical and chemical environment around the tissue or cell by the cell culturing means, (C) observing a first physical and chemical property of the tissue or cell in the first physical and chemical environment by the observing means, (D) changing the first physical and chemical environment to a second physical and chemical environment by the environment conditioning means, (E) observing a second physical and chemical property of the tissue or cell in the second physical and chemical environment by the observing means, and (F) comparing the first physical and chemical property of the tissue or cell with the second physical and chemical property of the tissue or cell by the comparing means.

Growing community of inventors

Katano, Japan

Hirokazu Sugihara