Hitachi, Ltd.

Mitsubishi Electric Corporation

Mitsubishi Denki Kabushiki Kaisha

The University of Tokyo

Hirokazu Nishida

Seiichi Mimura

Takafumi Kawasaki

Maiko Tanabe

Yasuhiro Yoshida

Takashi Ohshima

Kazuhiko Ishihara

Yoshizumi Ishino

Teruo Kohashi

Tomohiro Konno

Takao Matsumoto

Hisashi Tominaga

Kosuke Morikawa

Hirozoh Tsuruta

Masayuki Hamayasu

The present invention provides a method of producing a microorganism having an ability of assimilating a first factor including: a step of subjecting a microorganism to random mutagenesis; and a step of culturing the microorganism in the presence of a highly concentrated first factor and then selecting the grown microorganism.

Method of screening CO2-assimilating microorganism

An object of the present invention is to provide, as a method for immobilizing a protein molecule, protein immobilization method and means in which orientation of a molecule to be immobilized can be controlled, the molecule can be stably immobilized without a complicated process and a chemical group used for immobilization does not affect the activity and the function of the protein. Specifically, the present invention relates to a method for immobilizing a molecule including the steps of forming a labeled molecule by attaching a label peptide sequence comprising a hydroxyl group-containing amino acid to a molecule; and bringing a molecule having a phenylboronic acid group into contact with the labeled molecule, to capture the labeled molecule by the molecule having a phenylboronic acid group.

Oligopeptide sequence specifically bonding to phenylboronic acid group

To provide a multi-wire saw that, at the start of cutting of an ingot, prevents a wire from being displaced in grooves of guide rollers due to the wire being lifted. A wire is wound around a plurality of wire guide rollers to be positioned in a feeding direction of an ingot, and in this state, a wire-lifting restraining member that is a body of rotation and restrains the wire from being lifted by being brought into contact with the wire is disposed near the wire guide rollers.

Multi-wire saw and method for cutting ingot

Thermostable DNA ligases with enhanced DNA binding activity and reaction activity are obtained. These modified thermostable DNA ligases having enhanced DNA binding activity compared to the wild type can be obtained by substituting a negatively charged amino acid (for example, the amino acid corresponding to the aspartic acid at position 540 of SEQ ID NO: 1) present at the N-terminal side of the C-terminal helix moiety of thermostable DNA ligases from thermophilic bacteria, hyperthermophilic bacteria, thermophilic archaea, or hyperthermophilic archaea, with a non-negatively charged amino acid (for example, alanine, serine, arginine, or lysine).

Heat-resistant DNA ligase with high reactivity

The invention is a slurry for slicing a silicon ingot, containing a basic material, such as an alkali metal hydroxide, abrasive powder and water, in which the slurry contains the basic material in an amount of from 2 to 6% by mass and glycerin in an amount of from 25 to 55% by mass, based on a total mass of components of the slurry excluding the abrasive powder.

Slurry for slicing silicon ingot and method for slicing silicon ingot using the same

Disclosed is a modified hyperthermophilic DNA ligase having improved DNA binding ability and reactivity. The modified hyperthermophilic DNA ligase has an amino acid sequence corresponding to the amino acid sequence of a heat-resistant DNA ligase derived from a thermophilic bacterium, a hyperthermophilic bacterium, a thermophilic archaebacterium, or a hyperthermophilic archaebacterium, except with at least two of the charged amino acids in the C-terminal helix region each being substituted by alanine, threonine, or serine residues.

Highly efficient hyperthermophilic DNA ligase

It is intended to obtain DNA ligase improved in binding ability and reactivity with DNA, particularly thermostable DNA ligase improved in binding ability and reactivity with DNA. The present invention provides a DNA ligase mutant improved in binding ability and reactivity with DNA, which is obtained by partially or completely deleting a C-terminal helix portion of DNA ligase. Particularly, the mutant is derived from a thermostable bacterium.

DNA ligase mutants

Chirality distribution in the molecular structure of protein or the like and magnetic domain structure are analyzed with high resolution less than 10 nm. A transmission electron microscope equipped with a spin-polarized electron source is used for holography observation. The phase of transmission spin-polarized electrons changes due to the existence of chirality structure or magnetization in a sample, which is observed as an interference pattern phase shift in holography measurement.

Transmission electron microscope

A multi-wire saw according to the present invention cuts a workpiece while supplying a slurry containing an alkali or mixed acid to a cutting interface between the workpiece and a wire, and includes: a storage tank with a heating mechanism for storing and heating the slurry; a thermal insulating pipe for transporting the slurry sent with a pump from the storage tank with a heating mechanism to a position before a position where the wire is incorporated into the workpiece, while keeping the slurry at a predetermined temperature; a thermostat for keeping a temperature in a vicinity of the workpiece fixed to a stage at the predetermined temperature; and a wire heating mechanism for heating the wire to the predetermined temperature. Consequently, the cutting resistance decreases during cutting machining of a silicon ingot, and the variation in the cutting resistance also decreases, whereby a high-quality wafer can be obtained at a high efficiency and a low cost.

Growing community of inventors

Kokubunji, Japan

Hirokazu Nishida