Genedesign, Inc.

National Institute of Advanced Industrial Science and Technology

The University of Tokyo

Nof Corporation

Chiba University

Hirokazu Nankai

Takeshi Haraguchi

Hideo Iba

Hideaki Sato

Satoshi Inoue

Mutsuo Tanaka

Teiichi Murakami

Kohei Himeno

Provided is a novel technique for treating cancer using structurally-reinforced S-TuD. Provided are: a composition for the prevention or treatment of tumors, said composition comprising an miRNA inhibitory complex including RNA of analog thereof; and a method for preventing or treating tumors using said composition. The miRNA inhibitory complex preferably includes at least one double-stranded structure and an miRNA-binding sequence. Two strands of the miRNA binding sequence preferably bind individually to two strands on at least one end of the double-stranded structure. According to some of the aspects of the present invention, there is provided a delivery system for delivering such an miRNA inhibitory complex.

Technique for treating cancer using structurally-reinforced S-TuD

The present invention pertains to a method for synthesizing a ribonucleic acid H-phosphonate monomer, and a method for performing oligonucleotide synthesis in which said monomer is used. The present invention pertains to a method for manufacturing an inexpensively manufacturable H-phosphonate nucleoside derivative in which selective protection is provided to position 2' of a ribonucleoside monomer required in RNA oligonucleotide synthesis. The present invention is characterized in that: hydroxyl groups in position 2&#x2032; and position 3&#x2032;, which have slightly different reactivity, are caused to react with an aromatic acyl halide at low temperature to selectively esterify position 2&#x2032;; and subsequently the hydroxyl group at position 3&#x2032; in one pot is captured by a phosphityl group to prevent position 2&#x2032; and position 3&#x2032; transfer of the acyl group.

Method for synthesizing ribonucleic acid H-phosphonate monomer, and oligonucleotide synthesis in which said monomer is used

The present invention pertains to the improvement of a miRNA inhibitor (a synthesized Tough Decoy (S-TuD)). The present invention provides a miRNA inhibitory complex including RNA or an analog thereof, wherein the RNA inhibitory complex includes at least one double-stranded structure and a miRNA binding sequence, each of two strands of the miRNA binding sequence being bound to two strands of at least one end of the double-stranded structure, and the miRNA inhibitory complex further includes at least one crosslinked nucleic acid.

Structurally-enhanced miRNA inhibitor S-TuD

Growing community of inventors

Osaka, Japan

Hirokazu Nankai