Hitachi-High-Technologies Corporation

Kioxia Corporation

Hitachi High-Tech Corporation

Hirokazu Kato

Tomohiro Shoji

Satoshi Takahashi

Takanobu Haga

Nobutaka Kumazaki

Tsuyoshi Sonehara

Junji Shiokawa

Tomoyuki Sakai

Akihito Nishizawa

Akira Maekawa

Takuya Matsui

Junji Ishizuka

Ryoichi Suzuki

According to one embodiment, a wiring fabrication method includes pressing a first template including a first recessed portion and a second recessed portion provided at a bottom of the first recessed portion against a first film to form a first pattern including a first raised portion, corresponding to the first recessed portion, and a second raised portion, corresponding to the second recessed portion. The second raised portion protrudes from the first raised portion once formed. After forming the first pattern, a first wiring, corresponding to the first raised portion, and a via, corresponding to the second raised portion, is formed using the first pattern.

Wiring formation method, method for manufacturing semiconductor device, and semiconductor device

A multicolor fluorescence analysis device is provided. The device includes a first fiberoptic plate for guiding light including fluorescence emitted from a sample as a result of irradiation of excitation light and emitting the same from a first emission part. The device includes a second fiberoptic plate for receiving light emitted from the first emission part at a second incidence part, guiding the same, and emitting the same from a second emission part. The device includes a single multilayer dielectric interference film filter that is provided on an end surface of the second emission part, transmits at least a portion of the fluorescence, and transmits light of a plurality of transmission wavelength bands that do not include the excitation wavelength bands. The device includes a two-dimensional detection unit that is disposed so as to be adhered to the multilayer dielectric interference film filter.

Multicolor fluorescence analysis device

A multicolor fluorescence analysis deviceis for detecting fluorescence emitted, as a result of excitation light irradiation, from a plurality of types of fluorophores included in a sample s, and is provided with an irradiation optical unitfor irradiating light emitted from a light sourceonto a sample s as excitation light, a fluorescence condensation unithaving a fluorescence filterthat transmits light emitted from the sample s and transmits light of transmission wavelength bands different from the excitation wavelength bands, and a two-dimensional detectorthat has a plurality of types of transmission filtersfor transmitting prescribed wavelengths of light and detects the intensity of the light of the prescribed wavelength for each transmission filter, and the light emitted from at least two fluorophores from among the plurality of types of fluorophores is detected simultaneously and the fluorophore types are identified accordingly.

By slowing down the passing velocity of a DNA molecule in a nanopore, the accuracy of the reading of a nucleotide sequence of DNA is improved. A small temperature difference is introduced between a DNA molecule having an asymmetric and periodic structure and a nanopore membrane that carries the DNA molecule, whereby the DNA molecule that passes through a nanopore can move in one direction and the passing velocity of the DNA molecule in the nanopore can be controlled and reduced. In this manner, the accuracy of the analysis of a nucleotide sequence can be improved. Furthermore, it becomes possible to dissociate double-stranded DNA into single-stranded DNA molecules by the action of temperature and subject the single-stranded DNA molecules to a measurement selectively. Furthermore, it also becomes possible to select the polarity of a DNA molecule and subject the DNA molecule to a measurement.

Analysis device

The present invention has an object to provide a method for efficiently detecting an image with a smaller number of pixels. The invention relates to fluorescence analysis which uses a substrate having a plurality of regions for being capable of immobilizing biologically-related molecules in positions of lattice points of a lattice structure, and which causes the fluorescence from a certain lattice point to be wavelength-dispersed in a direction other than the direction toward the adjacent closest lattice point. According to an embodiment, for example, the number of pixels of a two-dimensional sensor required for fluorescence analysis of the regions with the biologically-related molecules immobilized can be set to several hundred times to fifty times smaller than that in the conventional case without degrading the measurement accuracy. This can achieve the improvement of throughput, reduction in price, and/or improvement of the operability of an analyzing device.

Fluorescence analyzing device and fluorescence analyzing method

A device and method for fluorescence observation have good operability, high sensitivity, and high acid reliability. The device is used for fluorescence observation using evanescent light. The angle of incidence of the excitation light is adjusted so that the excitation light is totally reflected from the surface of a substrate irrespective of the angle of the substrate surface. The method includes a step of shining the excitation light on the observation substrate while continuously varying the angle of the excitation light with respect to the observation substrate. In addition, the method includes a step of sensing the shone excitation light via optical sensors, and a step of setting the angle of total reflection according to the result of the sensing by the optical sensors. In the present device and method, the direction in which the shone excitation light travels varies with the angle of incidence.

Total internal reflection fluorescence (TIRF) observation device

A fluorescent detection apparatus relates to an analysis technique for qualitatively detecting or quantifying biomolecules by producing an evanescent field on a surface of a substrate, exciting fluorescently labelled biomolecules on the substrate surface in the evanescent field, and detecting the resultant fluorescent light emitted from the biomolecules. The fluorescent detection apparatus has a configuration in which a well is provided in a surface opposing to a sample substrate of a prism, the well is filled with a matching liquid, and the matching liquid is filled between the sample substrate and the prism, thereby improving operability and providing a stable evanescent field.

Growing community of inventors

Tokyo, Japan

Hirokazu Kato