Nisshin Seifun Group Inc.

Incorporated Administrative Agency National Agriculture and Food Research Organization

Hirohito Yamakawa

Katsuyuki Hayakawa

Akihiro Hino

Takashi Kodama

Mayu Iida

Satomi Nozaki

Eriko Suzuki

Kiyoko Miyatake

A circular DNA is provided comprising endogenous DNA common to both genetically modified wheat and non-genetically modified wheat along with one or more pieces of DNA each having a sequence present specifically in a strain of genetically modified wheat. Also provided is a method for determining a mix rate of genetically modified wheat in a test sample.

Method for detecting and quantifying endogenous wheat DNA sequence

A method of testing the presence/absence of wheat in a food by performing PCR with the use of primers which have been designed on the basis of data obtained from a part of a gene of wheat. This method is highly useful in detecting a trace component contained in a food or identifying a harmful allergen of wheat.

Method of testing wheat

A method for detecting the presence/absence of a specific substance in foods by performing PCR using primers which are designed on the basis of data obtained from a part of a gene of the specific substance. This method is highly useful in detecting a trace component contained in a food or identifying a specific harmful allergen.

Method of testing food

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Fujimino, Japan

Hirohito Yamakawa