Fuji Photo Film Company, Limited

Fujifilm Corporation

The University of Tokyo

Fujilfilm Corporation

Hirohiko Tsuzuki

Yoshio Inagaki

Hitoshi Shimizu

Katsuaki Muraishi

Tatsuo Fujikura

Yoshiyuki Kunuki

Masayasu Konishi

Kouji Kuruma

Koichi Minami

Akiko Matsuura

Yoshiharu Yabuki

Hisashi Okamura

Itsuo Fujiwara

Kazuhiro Aikawa

Satoru Toda

Koji Kuruma

Makoto Kato

Tadashi Ito

Hisashi Okada

Katsumi Kobayashi

Hiroshi Ueda

Seiji Yamashita

Masaki Noro

Toyohisa Oya

Naoyuki Hanaki

Yasunori Ichikawa

Fumiko Shiraishi

Tetsuo Kurahashi

Masaki Ihara

Hidenori Akutsu

An antibody-fragment-immobilizing substrate includes a substrate and at least one set of antibody fragments, wherein the antibody fragments of each set includes at least two types of separate antibody fragments that are capable of recognizing one type of antigen and that are independently immobilized on the substrate in a positional relationship that allows each of the antibody fragments in one set to bind to the same antigen.

Immobilization substrate and method for producing the same

A first reactant, which is provided with a reaction site for specific binding with an analyte, and a fluorescent label site, and a second reactant, which is provided with a reaction site for specific binding with the analyte, and a fluorescence recognition site for recognizing fluorescence produced by the fluorescent label site of the first reactant, are respectively fixed onto a support such that the first reactant and the second reactant have a positional relationship adapted for the binding with the analyte.

Carrier for analysis of an analyte and process for producing the same

A complex, which has been formed by at least two pieces of a ligand and a first metal ion, is bound with a carrier. A second metal ion is then added onto the carrier, a new complex being thereby formed. A substance that contains a Group-15 or Group-16 atom, which has metal coordinating capability, is then fixed to the first metal ion and the second metal ion.

Process for producing a carrier

An object of the present invention is to suppress variations in measurement values when measuring a specific binding reaction between a physiologically active substance and a tested substance using a surface plasmon resonance measurement device, so that binding detection data with high reliability is obtained. The present invention provides a method for measuring a change in surface plasmon resonance, which comprises: using a surface plasmon resonance measurement device comprising a flow channel system having a cell formed on a metal film and a light-detecting means for detecting the state of surface plasmon resonance by measuring the intensity of a light beam totally reflected on the metal film; and exchanging the liquid contained in the above flow channel system, wherein a major axis of the metal film is 0.1 &#x3bc;m or more and 100 &#x3bc;m or less; a molecule interacting with an analyzed molecule is immobilized on the surface of the metal film; the distance between metal films is 200 &#x3bc;m or more and 10 mm or less; and the molecule interacting with the analyzed molecule is not immobilized on any parts other than the metal films.

Method for measuring surface plasmon resonance

It is an object of the present invention to provide a method for analyzing the interaction of a test substance with a ligand by analyzing a phenomenon whereby multiple test substances are simultaneously adsorbed to a ligand. The present invention provides a method for analyzing the interaction of test substances with a ligand by measuring the change in the surface plasmon resonance using a surface plasmon resonance measurement device wherein the above-described method comprises: supplying a solution containing two or more types of test substances after supplying a solution containing no test substances; measuring the change in the surface plasmon resonance; and analyzing the difference between the change in the surface plasmon resonance that is predicted from the adsorption rate constant (ka) and dissociation rate constant (kd) of each of the test substances that had previously been measured and the change in the surface plasmon resonance that has actually been measured.

Method for analyzing test substance by surface plasmon resonance analysis

An object to be solved by the present invention is to determine the dissociation constant of an analyte molecule immobilized on a metal surface and a molecule that interacts therewith in surface plasmon resonance (SPR) analysis via an analytical method that produces a low noise level (i.e., a noise width of a reference chip), small baseline fluctuations (i.e., signal changes of a reference chip), and highly reliable results of measurement. The present invention provides a method for determining a dissociation constant of an analyte molecule immobilized on a metal surface and a molecule that interacts therewith, wherein changes in surface plasmon resonance signals are measured by using a surface plasmon resonance measurement device comprising a flow channel system having a cell formed on a metal film and a light-detecting means for detecting the state of surface plasmon resonance by measuring the intensity of a light beam totally reflected on the metal film; wherein a change in surface plasmon resonance signals is measured in a state where the flow of the liquid has been stopped, after the liquid contained in the above flow channel system has been exchanged; and wherein the dissociation constant is determined based on the results of measurement of signal changes.

Method for measuring dissociation constant by surface plasmon resonance analysis

It is an object of the present invention to provide a measurement method which can suppress noise when a specific binding reaction between a physiologically active substance and a test substance is measured using a surface plasmon resonance measurement device. The present invention provides a method for measuring a change in surface plasmon resonance which comprises: using a surface plasmon resonance measurement device comprising a metal film, a light source for generating a light beam, an optical system for allowing the above-described light beam to enter the interface of the metal film so that total reflection conditions can be obtained at the interface thereof and so that various incidence angles can be included, a flow channel system comprising a cell formed on the above-described metal film, and a light-detecting means for detecting the state of surface plasmon resonance by measuring the intensity of the light beam totally reflected at the above interface, and exchanging the liquid contained in the above flow channel system with another liquid, wherein the liquid in the above-described flow channel system is exchanged with another liquid obtained by eliminating insoluble matters from a measurement solvent, and the change in surface plasmon resonance is then measured in a state where the flow of the liquid has been stopped.

An object of the present invention is to suppress the noise width of a reference cell during measurement and the base line fluctuation. The present invention provides a method for measuring a change in surface plasmon resonance, which comprises: using a surface plasmon resonance measurement device comprising a flow channel system having a cell formed on a metal film and a light-detecting means for detecting the state of surface plasmon resonance by measuring the intensity of a light beam totally reflected on the meal film; and exchanging the liquid contained in the above flow channel system, wherein the above method is characterized in that a change in surface plasmon resonance is measured in a state where the flow of the liquid has been stopped, after the liquid contained in the above flow channel system has been exchanged.

An object of the present invention is to construct a culture device optimized for culturing animal cells. The present invention provides a device for culturing cells which comprises at least one water-containing polymer gel film for adhering animal cells onto at least one surface of the film, and has a structure capable of supplying different liquids to both sides of the film.

Device and method for culturing cells

The ultrasonic scatterer of the invention comprises gas-containing particles having an average particle size of 0.01 &#x3bc;m to 10 &#x3bc;m, the ultrasonic imaging method of the invention comprises transmitting an ultrasonic wave continuing for ten cycles or more; transmitting an ultrasonic wave continuing for four cycles or more and less than ten cycles after a predetermined period passes, and the ultrasonic imaging apparatus of the invention comprises transmitting means for sending a driving signal to the ultrasonic probe so as to transmit, to a subject, an ultrasonic wave continuing for four cycles or more and less than ten cycles after a predetermined period passes subsequently to transmitting, to the subject, of an ultrasonic wave continuing for ten cycles or more.

Ultrasonic scatterer, ultrasonic imaging method and ultrasonic imaging apparatus

A carrier for cell culture comprising a carrier having a cationic group and polypeptide-modified portions formed on a surface of the carrier in a sea-island structure, and a carrier for cell culture comprising a water-containing gel comprising alginic acid, wherein a surface of the carrier is coated with collagen, and wherein the collagen is bound to a surface of the water-containing gel by means of chitosan.

Carrier for cell culture

Sensor chip for biochemical analyzer

Prism for sensor chip for biochemical analyzer

Growing community of inventors

Kanagawa, Japan

Hirohiko Tsuzuki