Kyoto University

Japan Science and Technology Agency

Massachusetts Institute of Technology

Ips Academia Japan, Inc.

Hirohide Saito

Kei Endo

Tan Inoue

Seiya Takahashi

Shunichi Kashida

Kenji Miki

Kenichi Yoshikawa

Ayako Yamada

Yusho Kato

Toshiki Taya

Toru Yamanaka

Darrell J Irvine

Hideyuki Nakanishi

Ron Weiss

Yoshinori Yoshida

Yi Kuang

Tasuku Kitada

Hidetoshi Sakurai

Liliana Wroblewska

Maria Hottelet Foley

Karin Hayashi

Kaoru Richard Komatsu

Katie Bodner

Tyler Wagner

Takahiko Sato

Velia Siciliano

Jacob Becraft

Satoru Takayama

Shota Katayama

Callum Parr

Tetsuhiro Kobayashi

Yoshihiko Fujita

Tomoaki Hara

Eriko Osada

Hara; Tomoaki

An RNA probe containing RNA functional structural units is prepared by the following steps: (1) recognizing one or more stem structures contained in the RNA based on RNA sequence information; (2) extracting a motif region with reference to the one or more recognized stem structures; (3) adding an assistive stem region to the extracted motif region; and (4) adding a barcode region, which represents a complementary sequence to a DNA barcode sequence, to the assistive stem region. Also provided is a method for detecting a protein-binding RNA by using an RNA probe containing RNA functional structural units.

RNA structure library

Engineered synthetic RNA-based genetic circuits are provided that are regulated exclusively at the post-transcriptional level.

RNA-based logic circuits with RNA binding proteins, aptamers and small molecules

An mRNA forcibly expresses a protein gene in response to a miRNA, and a method for forcibly expressing the same, are provided. An artificial mRNA comprising a sequence encoding a protein gene, a miRNA target sequence linked to the 3'-terminal side of a Poly A sequence, and a translational repression sequence linked to the 3&#x2032;-terminal side of the miRNA target sequence; and a method for expressing a protein gene in response to the expression of a miRNA, comprising a step of introducing the artificial mRNA into a cell.

Method for expressing protein gene in response to expression of miRNA

Interaction with a protein is detected by using an RNA probe containing the following sequences;

RNA microarray for detecting interaction between protein and RNA containing a higher-order structure

[Problem to be Solved] To provide a compound for removing pluripotent cells from a cell population potentially containing the pluripotent cells. [Solution] A polyphenylalanine derivative is contacted with a cell population of interest.

Compound for identifying pluripotent cells

The present invention aims to provide a method of sorting a skeletal muscle progenitor cell from a cell population containing the skeletal muscle progenitor cell. The above-mentioned problem is solved by providing a step of introducing miRNA-responsive mRNA into a cell population. The miRNA-responsive mRNA contains (i) a nucleic acid having a sequence specifically recognized by miRNA specifically expressed in a skeletal muscle progenitor cell, and (ii) a nucleic acid containing a sequence encoding a marker protein.

Method for selecting skeletal muscle progenitor cell

A method for extracting differentiated cells from a cell population comprising undifferentiated cells after induction of the differentiation of pluripotent stem cells. A method for extracting differentiated cells from a cell population, comprising the following steps: (1) a step of introducing, into a cell population, mRNA comprising a marker gene operably linked to the target sequence of miRNA specifically expressed in pluripotent stem cells; and (2) a step of extracting cells in which the marker gene has been translated.

Method for extracting differentiated cells

A method for distinguishing living cells in a living state with high accuracy. A method for distinguishing a desired cell type from a cell group comprising two or more types of cells, using the expression of miRNA as an indicator, wherein the method comprises the following steps:

Method for determining desired cell type using expression of miRNA as indicator

An object of the present invention is to provide a novel method for sorting cardiomyocytes. Another object of the present invention is to provide a method for producing high-purity cardiomyocytes and a kit used therefor. The present invention provides a method for sorting cardiomyocytes, comprising a step of introducing miRNA-responsive mRNA into a cell group, wherein the miRNA-responsive mRNA consists of a sequence comprising the following (i) and (ii):

Method for sorting cardiomyocytes

An object of the present invention is to provide a method for increasing the purity of a type of tissue cell such as an endothelial cell, a hepatocyte, or an insulin-producing cell. The present invention solves the problem by providing a method comprising a step of introducing, into a cell population, an mRNA comprising a nucleic acid sequence recognized by an miRNA specifically expressed in endothelial cells, hepatocytes, or insulin-producing cells.

Method for sorting tissue cells

Provided is a method for continuously visualizing and accurately determining a living cellular state per se. Disclosed are: a vector comprising 5'- and 3&#x2032;-end nucleic acid sequences for integration and a reporter gene sequence positioned between the 5&#x2032;- and 3&#x2032;-end nucleic acid sequences for integration; a gene introduction agent comprising a first vector and a second vector; a cell comprising the vector or the agent; and a method for determining a cellular state by using the cell.

Vector and gene introduction agent for monitoring and visualizing cell differentiation using expression of micro RNA as indicator and monitoring and visualizing method using thereof

A translational control method using an RNA-protein interaction motif is provided. The method comprises a step of introducing an mRNA having: a 5'UTR regulation structure comprising: (1) a cap structure at the 5&#x2032; terminus, (2) a spacer positioned on the 3&#x2032; side of the cap structure, and (3) one or more RNA motifs positioned on the 3&#x2032; side of the spacer, which comprises an RNA-protein interaction motif-derived nucleotide sequence or a variant thereof; and a nucleotide sequence encoding a target protein gene on the 3&#x2032; side of the 5&#x2032;UTR regulation structure, into a cell in the presence of a protein specifically binding to the RNA motifs, wherein a translational level is decreased as the number of bases of the spacer decreases, and the translational level is decreased as the number of the RNA motifs increases.

Translational control system using RNA-protein interaction motif

An object of the present invention is to provide a translationally regulatable mRNA which has wider application and can perform specific ON-OFF regulation, an RNA-protein complex specifically bound to the mRNA, and a translational regulatory system. The present invention provides an mRNA having an RNA-protein complex interacting motif-derived nucleotide sequence 5' to the ribosome-binding site or within the 5&#x2032; region of the open reading frame, and an mRNA having a nucleotide sequence complementary to an RNA-protein complex interacting motif-derived nucleotide sequence 5&#x2032; to the ribosome-binding site or within the 5&#x2032; region of the open reading frame.

Protein-responsive translational regulatory system using RNA-protein interacting motif

Growing community of inventors

Kyoto, Japan

Hirohide Saito