Toyo Kohan Co., Ltd.

Yamaguchi University

Hirofumi Yamano

Michifumi Tanga

Ryouichi Tsunedomi

Mitsuyoshi Ohba

Hiroshi Okamura

Mitsuyoshi Oba

Koichi Hirayama

Shoichi Hazama

Yoshihiko Hamamoto

Shuichi Kamei

Hiroaki Nagano

Shuuichi Kamei

Yukiha Ishikawa

Toshiya Tsuda

Yoshiaki Yamamoto

Akane Ito

Yusuke Fujita

Hideyasu Matsuyama

Teruhisa Icihara

Shyuichi Kamei

It is intended to conveniently determine the pharmacokinetics of axitinib and to predict the therapeutic effect of axitinib. The present invention provides a method for determining the pharmacokinetics of axitinib, comprising the step of calculating a predicted pharmacokinetic parameter of axitinib using specific gene polymorphisms and background factors regarding a test subject.

Method for determining pharmacokinetics of axitinib and method for predicting therapeutic effect of axitinib based on pharmacokinetics of axitinib

In the case of using a blocking nucleic acid to prevent non-specific hybridization of a target nucleic acid with a nucleic acid probe, further excellent efficiency of detecting the target nucleic acid is achieved. A buffer composition used in hybridization of a target nucleic acid with a nucleic acid probe, wherein the buffer composition for hybridization contains a blocking nucleic acid comprising a nucleotide sequence complementary to a region comprising at least a non-detection target nucleotide in a non-target nucleic acid, in a concentration of one or more times higher than the concentration of a nucleic acid in a nucleic acid mixture consisting of the target nucleic acid and the non-target nucleic acid.

Buffer composition for hybridization and hybridization method

A solid support for maintaining a uniform spot shape while improving the capacity for immobilization of nucleic acids upon spotting of nucleic acids onto a solid support is provided. A substrate has a base material, an electrostatic layer formed on the base material in order to electrostatically attract nucleic acids, and carboxyl groups formed on the electrostatic layer, wherein the ratio, (COO peak intensity)/(C&#x2014;C peak intensity) in the C1s spectra obtained by X-ray photoelectron spectrometry (XPS) performed for the surface of the substrate ranges from 0.10 to 0.20. The solid support for immobilizing nucleic acids is produced by active esterification of carboxyl groups of the substrate.

Carrier for holding nucleic acid

The present invention relates to a unit to be used for detection of interactions of biologically relevant molecules using a carrier on which the biologically relevant molecules are immobilized, comprising:

Method and unit for detection of interactions of biologically relevant molecules

It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.

Solid support having electrostatic layer and use thereof

A method for detecting hybridization between a probe polynucleotide and a target polynucleotide on a microarray is presented. The method may be used to determine the accuracy of hybridization and possible causes of inaccuracy, such as insufficient washing or deterioration of the microarray.

Method for detecting a position of probe on microarray

Described is a means for objectively determining hybridization failure in addition to performance degradation, insufficient washing and the like in a microarray. In particular a method for detecting the hybridization between a probe polynucleotide and a target polynucleotide by using a microarray is presented.

Detection method for microarray

Growing community of inventors

Yamaguchi, Japan

Hirofumi Yamano