Kyoto University

Shimadzu Corporation

Osaka University

Nipro Corporation

Hirofumi Suemori

Norio Nakatsuji

Eihachiro Kawase

Takamichi Miyazaki

Takashi Suzuki

Shinya Yamanaka

Kiyotoshi Sekiguchi

Motonari Uesugi

Masato Nakagawa

Kazumitsu Ueda

Atsushi Taguchi

Sugiko Futaki

Ryo Tomii

Yukimasa Taniguchi

Yuto Fujibayashi

Maria Hayashi

Tsuneo Takahashi

Nao Hirata

Shinichiro Chuma

Asako Murata

Young-Tae Chang

Kaori Yamauchi

Provided is a cell culture method comprising the step of culturing cells using a medium containing a laminin fragment having integrin binding activity, the method not comprising the step of coating a culture vessel with a laminin or a laminin fragment before seeding the cells in the culture vessel. The cell culture method of the present invention uses a smaller amount of a laminin fragment and still achieves a comparable culture efficiency as compared with the conventional cell culture method that uses a culture vessel precoated with a laminin or a laminin fragment.

Cell culture method using laminin fragment-containing medium

A biological sample or drug stored inside a frozen storage container is not susceptible to contamination. The frozen storage container includes a container body with an internal space, and a partition wall which divides the internal space at least into a first space and a second space. In the container body, the first space is liquid-tightly sealed, the second space has an opening which is in communication with the outside, and the second space is liquid-tightly sealable by welding the container body. A frozen storage container system includes the frozen storage container; and a needle member which includes a needle tip portion capable of being inserted into the internal space of the container body and capable of piercing the partition wall, and has a flow path which is opened at the needle tip portion and is opened at a base end portion opposite to the needle tip portion.

Frozen storage container and frozen storage container system

In the present invention, for test cells which are either stem cells whose state of differentiation is unknown or cells obtained from stem cells by differentiation induction, an LC-MS or GC-MS analysis is performed on culture supernatants collected from a culture dish of the test cells and a culture dish of control cells whose state of differentiation is known, and the state of differentiation of the test cells is assessed based on the amount, determined as a result of the aforementioned analysis, of at least one compound selected from the group of putrescine, kynurenine, cystathionine, ascorbic acid, riboflavin, pyruvic acid, serine, cysteine, threonic acid, citric acid, and orotic acid in both the culture supernatant of the test cells and the culture supernatant of the control cells.

Method for assessing state of differentiation of cells

A method for sorting pluripotent cells using a compound which is eliminated from the pluripotent cells through the MDR1 transporter.

Method for sorting of pluripotent cells

The present invention provides a culture substrate which enables maintenance culture of human pluripotent stem cells in a pluripotent state under a feeder-free culture environment, and a culture method of human pluripotent stem cells using the culture substrate. By seeding human pluripotent stem cells dissociated into single cells at a cell density of 4&#xd7;10to 10&#xd7;10cells/cmonto a culture substrate coated with human laminin &#x3b1;5&#x3b2;1&#x3b3;1 E8 fragment or human laminin &#x3b1;3&#x3b2;3&#x3b3;2 E8 fragment preferably at a concentration of 0.5 to 25 &#x3bc;g/cm, the human pluripotent stem cells can be rapidly expanded in a pluripotent state.

Culture substrate for human pluripotent stem cells and use thereof

Growing community of inventors

Kyoto, Japan

Hirofumi Suemori