Nikon Corporation

The University of Tokyo

Hirofumi Shiono

Takanori Ichiki

Ryo Kobayashi

Yasujiro Kiyota

Takayuki Uozumi

Nobuhiko Maiya

Taro Ueno

Shingo Ueno

Manish Biyani

Hisao Osawa

Naoto Nemoto

Takanori Akagi

Takashi Funatsu

Keiji Mitsui

The present invention provides a fluidic device in which solutions of different concentrations can be easily obtained. The fluidic device includes: a first substrate and a second substrate which are stacked in a thickness direction; an undiluted solution introduction flow path which has an undiluted solution introduction port and which is constituted of a groove part provided on at least one of the first substrate and the second substrate; a first circulation flow path which is constituted of a groove part having an annular shape and having a shared part that shares part of a flow path with the undiluted solution introduction flow path and a non-shared part which is not shared with the undiluted solution introduction flow path and which is connected to a diluting solution introduction port; a second circulation flow path which is provided independently of the first circulation flow path and which is constituted of a groove part having an annular shape and having a shared part that shares some flow path with the undiluted solution introduction flow path and a non-shared part which is not shared with the undiluted solution introduction flow path and which is connected to a diluting solution introduction port; and/or a third circulation flow path which is constituted of a groove part having an annular shape and having a shared flow path that shares part of a flow path with the first circulation flow path and a non-shared flow path which is not shared with the first circulation flow path and which is connected to a diluting solution introduction port, wherein the undiluted solution introduction flow path includes a valve at both ends of the shared part.

Fluidic device, system, and mixing method

A fluidic device includes a first circulation flow path and a second circulation flow path which circulate a solution containing a sample material, the first circulation flow path and the second circulation flow path share at least a part of the flow path, and at least one selected from the group consisting of a capture unit which captures the sample material, a detection unit which detects the sample material, a valve, and a pump is provided on the shared flow path.

Fluidic device, system, method of detecting sample material and method of purifying sample material

A fluidic device includes: a circulation flow path; and a capture part arranged on the circulation flow path and configured to capture a sample substance in a solution and/or a detection part arranged on the circulation flow path and configured to detect a sample substance in a solution. A method of capturing a sample substance that is bound to a carrier particle, using a fluidic device which includes a circulation flow path and a capture part arranged on the circulation flow path and configured to capture the carrier particle and in which the circulation flow path has two or more circulation flow path valves, includes: an introduction step of, in a state where the circulation flow path valve is closed, introducing a solution that includes a sample substance to at least one of partitions partitioned by the circulation flow path valve and introducing a solution that includes a carrier particle which is bound to the sample substance to at least another of the partitions; a mix step of opening all of the circulation flow path valves and circulating and mixing a solution in the circulation flow path; and a capture step of capturing the carrier particle by the capture part.

Fluidic device, system, and method

A device for measuring activity of cultured cells includes a position detecting unit specifying a position of a cell to be measured, a microchamber controlling unit disposing in the culture container a microchamber which surrounds the cell and forms a measurement space, the measurement space being minute with respect to a volume of the culture container, and a measuring unit measuring environmental factors contained in the measurement space.

Device for measuring activity of cultured cells, microchamber and method of measuring activity of cultured cells

The present invention relates to a nucleic acid linker for producing a complex of mRNA, and a protein or a peptide which is encoded by the mRNA, the linker comprising: a spacer portion at the 5'-terminal; a polynucleotide portion hybridizable with at least a part of a sequence of the mRNA; and an arm portion which has a connection portion for the protein or the peptide at the 3&#x2032;-terminal, in which the spacer portion, the polynucleotide portion, and the arm portion form a single strand, and in which the polynucleotide portion contains a photoreactive base derivative.

Nucleic acid linker

A method of analyzing an exosome is provided for detecting abnormality in a cell, including: (a) a step of preparing an exosome from a sample; (b) a step of bringing the exosome prepared in the step (a) into contact with a first antibody to a protein which exists on the surface of the exosome as an antigen and forming a first antibody-exosome complex; and (c) a step of measuring a zeta potential of the first antibody-exosome complex.

Exosome analysis method, exosome analysis apparatus, antibody-exosome complex, and exosome electrophoresis chip

A method for detecting a target nucleic acid, comprising: (a) contacting a nucleic acid sample comprising a target nucleic acid, comprising a first portion and a second portion, with: (i) a detection probe, wherein the detection probe is labeled with a labeling substance and comprises a nucleic acid sequence that forms a stem-loop structure and having a 5' protruding end or a 3&#x2032; protruding end that is capable of hybridizing to the second portion, and (ii) a capture probe comprising a nucleic acid sequence capable of hybridizing to the first portion, wherein the capture probe is immobilized to a substrate, under conditions to form a target nucleic acid-detection probe-capture probe complex by hybridizing the second portion to the detection probe and hybridizing the first portion to the capture probe; (b) ligating a first end of the detection probe with an end of the target nucleic acid and ligating a second end of the detection probe with an end of the capture probe; and (c) detecting the labeling substance of the nucleic acid-detection probe-capture probe complex formed on the substrate.

Nucleic acid detection method, detection probe, detection probe set, and nucleic acid quantification method

The present invention relates to a protein or peptide printing method, comprising (a) a step for preparing nucleic acids and a cell-free protein synthesis system in an engraved plate composed of microscopic grooves having a specific opening shape, (b) a step for superimposing a substrate on the engraved plate so as to contact a protein or peptide to be synthesized in the microscopic grooves, and (c) a step for synthesizing the protein or peptide from the nucleic acids using the cell-free protein synthesis system in the microscopic grooves, and immobilizing the protein or peptide on the substrate along the specific opening shapes of the microscopic grooves.

Protein or peptide printing method, protein array or peptide array, and functional protein or functional peptide identification method

A culture device containing a culture vessel for culturing a cell, in which the culture device includes a thermostatic vessel having a stacker containing a plurality of culture vessels for culturing a cell, and an observation portion arranged to be isolated from an atmosphere at inside of the thermostatic vessel and having an object lens, an object lens drive portion, and a camera portion to observe a cell at inside of the culture vessel installed at an observation position at inside of the thermostatic vessel.

Culture device

A transfer device for a culture vessel for transferring a culture vessel for culturing a cell, a culture device including the transfer device for a culture vessel, and a holder for a culture vessel for holding a culture vessel, in which the transfer device for a culture vessel includes a transferring unit transferring a culture vessel for culturing a cell, an inputting unit inputting a kind of the culture vessel, a speed setting unit setting a transfer speed of the culture vessel based on a kind of the culture vessel inputted to the inputting unit, and a controlling unit controlling a transfer speed of the transferring unit to be the transfer speed set by the speed setting unit.

Transfer device for culture vessel, culture device and holder for culture vessel

A protein-immobilizing solid phase is a protein-immobilizing solid phase comprising an mRNA-nucleic acid linker-protein complex, obtained by linking the mRNA and the protein encoded by that mRNA through the nucleic acid linker, immobilized on the solid phase, wherein the nucleic acid linker has a photocleavage site and a solid phase binding site.

Protein-immobilizing solid phase, polynucleotide-immobilizing solid phase, and nucleic acid recovery method

An automatic culture devicehaving an automatic quality determination system is equipped with an analysis program. The analysis programextracts cell characteristics from a captured image of a cell by driving a characteristic quantity extraction program, which is an image processing program for extracting characteristics (characteristic quantities) of this cell. The quality of the cell is then determined from the extracted characteristic or a combination of a plurality of characteristics by driving an identification programthat determines the quality of a cell. This allows cell quality determination to be automated.

Automatic system for judging quality of cell

An incubator including a temperature-controlled room adjusted to a predetermined environment condition for culturing a sample of an incubation container at inside of the temperature-controlled room, the incubator including a shifting mechanism for moving a position of the incubation container at inside of the temperature-controlled room by a motor, a temperature adjusting section for adjusting a temperature at inside of the temperature-controlled room, an operating information generating section for generating operating information with regard to a position of operating and a time period of operating the motor prior to operating the motor, an estimated variation outputting section for outputting an estimation variation of a temperature state by operating the motor based on the operating information, and a controlling section for controlling the temperature adjusting section to cancel a temperature change of an amount of the estimated variation in synchronism with operating the motor.

Growing community of inventors

Fujisawa, Japan

Hirofumi Shiono