Kaneka Corporation

Pfizer Corporation

Takasago International Corporation

Kanegafuchi Kagaku Kogyo Kabushiki Kaisha

Hirofumi Maeda

Kenji Inoue

Yoshihiko Yasohara

Ryoji Noyori

Nobutaka Tani

Akira Nishiyama

Kenichi Senda

Masaru Mitsuda

Nobuo Nagashima

Takaji Matsumoto

Akio Fujii

Masao Ando

Tatsuyoshi Tanaka

Masanobu Sugawara

Susumu Saito

Hiroshi Kita

Izuru Nagasaki

Hiroyuki Watanabe

Keishi Takatsu

Toshihiro Shikakura

Toshihide Fujii

A method for producing a ribosome display complex includes obtaining a ribosome complex including an unmodified polypeptide chain, an mRNA molecule and a ribosome by initiating translation of the mRNA molecule in a cell-free peptide synthesis system including the ribosome, and modifying the unmodified polypeptide chain by reacting a side chain reactive functional group in the unmodified polypeptide chain with a modifying reagent to produce a ribosome display complex including a modified polypeptide chain, the mRNA molecule and the ribosome. The unmodified polypeptide chain includes at least one reactive amino acid residue selected from the group consisting of a cysteine residue, a lysine residue, a histidine residue and a tryptophan residue. The at least one reactive amino acid residue includes the side chain reactive functional group, and the mRNA molecule includes a base sequence encoding an amino acid sequence of the polypeptide chain.

Ribosome display complex and production method therefor

The present invention relates to a method for producing a 2'-hydroxy-protected nucleoside derivative by reacting a ribonucleoside with an acylating or a carbamoylating reagent in the presence of a metal complex consisting of a copper compound and an optically active ligand. By the method according to the present invention, a 2&#x2032;-hydroxy-protected ribonucleoside derivative, which is an important intermediate for producing an oligonucleoside, can be easily produced with good regioselectivity from a nucleoside derivative of which 2&#x2032;,3&#x2032;-hydroxy groups are not protected.

2'-hydroxy-protected ribonucleoside derivative and production method thereof

Disclosed is a method for producing an alcohol from a lactone or a carboxylic acid ester, which enables to produce an alcohol from a lactone or a carboxylic acid ester under relatively mild conditions with high yield and high catalytic efficiency. This method also enables to produce an optically active alcohol from an optically active lactone or an optically active carboxylic acid ester. Specifically disclosed is a method for producing an alcohol by hydrogen reducing a lactone or a carboxylic acid ester in the presence of a catalyst containing ruthenium and a phosphine compound represented by the following general formula (1): wherein Rrepresents a spacer; R, R, R, R, Rand Rindependently represent a hydrogen atom, an alkyl group having 1-12 carbon atoms, an aryl group or a heterocyclic group; and R, R, R, R, Rand Rindependently represent an alkyl group having 1-12 carbon atoms, an aryl group or a heterocyclic group.

Method for producing alcohol by hydrogenating lactone and carboxylic acid ester in liquid phase

The present invention provides a method for easily producing an (R)-3-[4-(trifluoromethyl)phenylamino]-pentanoic acid amide derivative useful for an intermediate for pharmaceutical products, particularly an inhibitor of a cholesteryl ester transfer protein (CETP) from easily available raw materials. In the present invention, (S)-N-[4-(trifluoromethyl)phenyl]-3-hydroxypentanoic acid amide prepared from easily available raw materials leads a production of (R)-4-ethyl-1-[4-(trifluoromethyl)phenyl]-2-azetidinone to give (R)-3-[4-(trifluoromethyl)phenylamino]-pentanoic acid amide. Furthermore, (R)-4-ethyl-1-[4-(trifluoromethyl)phenyl]-2-azetidinone is reacted with a carbamic acid ester to give an (R)-3-[4-(trifluoromethyl)phenylamino]-pentanoic acid amide derivative.

Method for producing (R)-3-[4-(trifluoromethyl) phenylamino]-pentanoic acid amide derivative

Growing community of inventors

Takasago, Japan

Hirofumi Maeda