Mitsubishi Denki Kabushiki Kaisha

Savid Therapeutics Inc.

Mitsubishi Electric Corporation

Japan Science and Technology Corporation

The University of Tokyo

Daiichi Pharmaceutical Co., Ltd.

Perseus Proteomics Inc.

Celestar Lexico-sciences, Inc.

Celish Fd, Inc.

Japan Science and Technology Agency

Hirofumi Doi

Akira Sugiyama

Tatsuhiko Kodama

Tsuyoshi Inoue

Motomu Kanai

Yohei Shimizu

Eiichi Mizohata

Tatsuya Kawato

Tomohiro Meshizuka

Mutsuo Sekiya

Kenta Hatano

Hideaki Hiraki

Mari Takatsu

Noriaki Takasu

Akio Kanai

Masashi Wakisaka

Sotsuo Miyoshi

Naoki Miyamoto

Naoya Wada

Toshihiko Miyake

Yoshizumi Ishino

Takao Hamakubo

Tomohiro Yamamoto

Masahiro Matsuoka

Toshiyuki Umemoto

Katsuya Miki

Takakazu Tochimoto

Kouhei Tsumoto

Tatsuhiro Nakano

Chizuru Nagayoshi

Tadaaki Tokida

Satoshi Hihara

Takashi Kudou

Akira Kawamura

Shinya Hosogi

An object of this invention is to provide a streptavidin mutant reduced in affinity to the naturally-occurring biotin, and to provide a modified biotin which shows a high affinity to such streptavidin mutant reduced in affinity to the naturally-occurring biotin. This invention can provide a compound composed of a dimer of modified biotin, a streptavidin mutant, and usage of them.

Modified biotin, streptavidin mutant, and usage of them

An object of this invention is to provide a streptavidin mutant reduced in affinity to the naturally-occurring biotin, and to provide a modified biotin which shows a high affinity to such streptavidin mutant reduced in affinity to the naturally-occurring biotin. This invention can provide a compound composed of a dimer of modified biotin, a streptavidin mutant, angsd usage of them.

It is an object of the present invention to provide a mutant streptavidin with a reduced affinity for natural biotin, and also to provide a modified biotin having a high affinity for the mutant streptavidin with a reduced affinity for natural biotin. According to the present invention, there is provided a reagent kit for use in treatments or diagnoses, which comprises: (a) a mutant streptavidin with a reduced affinity for natural biotin or biocytin; and a modified biotin having a high affinity for the above-described mutant streptavidin.

Modified biotin, mutant streptavidin, and use thereof

A method for identifying the presence of a target gene mRNA is provided, which involves hybridizing one or more oligonucleic acid probes with the target gene mRNA expressed in a tissue sample, and detecting a low-molecular-weight compound label added to at least one of the bases of the oligonucleic acid probes. The oligonucleic acid probes are contacted with the tissue sample for hybridization with the target gene mRNA after pretreating (prehybridizing) one or more dummy oligonucleic acids with the tissue sample, or a mixture of the oligonucleic acid probes and the dummy oligonucleic acids is contacted with the sample tissue to hybridize the oligonucleic acid probes with the target gene mRNA.

RNA in situ hybridization

It is an object of the present invention to provide a mutant streptavidin wherein the immunogenicity (antigenicity) in mammals of a streptavidin is reduced. The present invention provides a mutant streptavidin, which comprises an amino acid sequence in which (a) the arginine residue at position 72 is substituted with another amino acid residue, and (b) any one or more of the tyrosine residue at position 10, the tyrosine residue at position 71, the glutamic acid residue at position 89, the arginine residue at position 91, and the glutamic acid residue at position 104 are substituted with other amino acid residues, with respect to the amino acid sequence of a core streptavidin as shown in SEQ ID NO: 2, and which has decreased immunogenicity as compared with that of a wild-type streptavidin.

Streptavidin having low immunogenicity and use thereof

An actuator having an output axis which is moved linearly by a rotatory force, and a boss for holding the above-mentioned output axis in such a way that the output axis can move linearly includes a connecting member for propagating the linear motion of the above-mentioned output axis to an external device. The above-mentioned connecting member is comprised of a joint connected to the above-mentioned output axis, and a link plate having an end rotatably connected to the above-mentioned joint, and another end rotatably connected to the external device. A mechanism for preventing rotation of the above-mentioned output axis is constructed of only a connecting portion for connecting the above-mentioned joint, the above-mentioned link plate, and the above-mentioned external device to one another.

Actuator having a rotation prevention link plate

The present invention is to construct a DNA replication reaction system which is excellent in versatility and is easily used. An amino acid sequence of a PCNA monomer which is one of factors involved in DNA replication is prepared so that amino acid residues causing mutual charge repulsion constitute a site which causes, when an N terminal region of the PCNA monomer and a C terminal region of another PCNA monomer act as an interface to form a multimeric complex, an intermolecular interaction of the monomers in an interface region of the monomers.

Mutant PCNA

A terminal structure for a vehicle-mounted motor includes a motor body having a motor terminalconnected to a coil, and a circuit bodyholding a circuit boardfor controlling a rotation of the motor body and is assembled in the motor body, wherein a stress relaxation sectionis provided in an intermediate of an intermediate conductor, in order to reduce stress acting on the intermediate conductorconnecting the motor terminaland the circuit boardand to thereby improve the conductive reliability of electrical joint.

Structure of motor terminal

The present application provides a method for predicting the functional site of a protein using data of the entire proteins of an organism of which genome data or cDNA data is known. More specifically, the present application provides a method for predicting a protein functional site, comprising the steps of calculating the frequency of occurrence of an oligopeptide in the entire proteins, calculating the value of each amino-acid residue contributing to the frequency of occurrence as the representative value of the function, and predicting the protein functional site by using the representative value of function as an indicator. The present also provides a system for predicting a functional site for automatically performing said methods. Additionally, the present application provides a method for preparing a function-modified protein comprising subjecting the amino-acid residues composing the functional site identified by the method described above to artificial mutation, and a novel thermophilic DNA polymerase prepared by the method.

Method and a system for predicting protein functional site, a method for improving protein function, and a function-modified protein

PAK4 and JIK, both of which bind to MKK7 and directly phosphorylate MKK7, were found in the present invention. The present invention provides an inhibitor of c-Jun phosphorylation caused by JNK3 and a method for inhibiting the same, and an agent for preventing and/or treating a disorder attributable to c-Jun phosphorylation caused by JNK3 and a method for preventing and/or treating the same, all of which comprise inhibiting one member selected from the following: the binding of PAK4 to MKK7, the phosphorylation of MKK7 by PAK4, the binding of JIK to MKK7, and the phosphorylation of MKK7 by JIK. Further, the present invention provides a method for identifying a compound that inhibits the binding of PAK4 to MKK7, the phosphorylation of MKK7 caused by PAK4, the binding of JIK to MKK7, or the phosphorylation of MKK7 caused by JIK, as well as the compound obtained thereby. Furthermore, the present invention provides a pharmaceutical composition containing an effective amount of at least one member selected from the group consisting of the aforementioned compound and the aforementioned inhibitor.

MKK7 activation inhibitor

An electrical actuator has a DC motor which is provided with a rotor having a permanent magnet for forming a plurality of magnetic poles around a periphery thereof and a stator having a plurality of energizing coils and being disposed around a periphery of the rotor to thereby generate a rotary force for the rotor. In mounting this DC motor on a heat-generating member which generates radiant heat, a member for shielding radiant heat from a heat-generating member is disposed between the DC motor and the heat generating member. In this manner, the radiant heat from the heat-generating member is prevented from reaching the DC motor, whereby the problems in the DC motor associated with the radiant heat can be prevented.

Electrical actuator

A brushless DC motor includes: a motor section which has a rotor, a stator and a housing; a control circuit section which is integrated with the motor section and separated from the motor section by a partition; and a casing member which covers the control circuit section to isolate the control circuit section from the external environment. A position detection means is disposed outside of a bearing member with respect to an axial direction.

Brushless DC motor

There is disclosed a stepping motor capable of increasing assembling work efficiency when the tooth of the gear to be driven provided to a driven member is connected to the tooth of the output shaft gear of the rotor of the stepping motor. The number of teeth for an output shaft gear is set to a predetermined ratio with respect to the number of magnetically stable points per rotation of the rotor, such that the gear to be driven can be held by a reference position stopper when a coil is electrified by a regulated electrification pattern. Thus, while the coil is electrified by the regulated electrification pattern, and the gear to be driven is held by the reference position stopper, the tooth respectively of the gear to be driven, and the output shaft gear are connected to each other. Therefore, these teeth are engaged with each other in a normal position with respect to a reference position.

Stepping motor having a predetermined number of teeth corresponding to magnetically stable points per rotor rotation

A stepping motor is provided with a plate spring for pressing a housing and a rotor against each other. One of the housing and the rotor is provided with a projection. Variations in position, which is inherent in position control by a related art stepping motor, due to lack of a retaining force subsequent to an initial position setting or due to variations in rotating speed of the motor, are eliminated. Thus the motor is suitably used for controlling of the position of, for example, an HID lamp unit.

Stepping motor for stable control of position of driven body

Growing community of inventors

Chiba, Japan

Hirofumi Doi