The Green Cross Corporation

Hirofumi Arimura

Tadakazu Suyama

Yahiro Uemura

Haruhide Kawabe

Hiroshi Morise

Satoshi Funakoshi

Masanori Nagai

Masayuki Nishida

Hiromichi Mukai

Hajime Horii

Muneo Tsujikawa

Kaoru Kobayashi

Takao Ohmura

Akinori Sumi

Wataru Ohtani

Koji Murakami

Kiyoshi Tsutsui

Ryuji Hiramatsu

Toshizumi Tanabe

Ken Okabayashi

Akimasa Ohmizu

Shusei Uno

Kazuo Ikegaya

Yukimitsu Nakagawa

Yutaka Ishida

Hiroshi Nakakubo

Yasuo Amatsuji

Kohji Murakami

Kazumoto Hirabayashi

Sadao Ueda

Masaaki Hirose

Hitoshi Minamino

Naofumi Hayasuke

Atusi Masaki

Teruo Kaneda

Takeshi Yamauchi

Tsutomu Kitagawa

Yoshiomi Okuno

Yoshitaka Sakanishi

Hazime Horii

A method for preparing foreign protein in yeast using an expression recombinant DNA comprising DNA encoding the serum albumin signal peptide adjacent to DNA encoding the foreign protein is disclosed.

Method of preparing foreign protein in yeast, recombinant DNA,

A CSF-1 gene promoter region is disclosed. This nucleotide base sequence is useful as a promoter for gene expression in animal cells.

CSF-1 gene promoter

A human prourokinase mutant in which the entire or a partial epidermal growth factor domain of human prourokinase is deleted or a partial epidermal growth factor domain of human prourokinase is replaced by one or more different amino acid residues, said mutant having a longer blood half-life than naturally occurring human prourokinase while retaining prourokinase enzymatic activity. In this human prourokinase mutant the region selected from the group consisting of: (a) from asparagine (10) to cysteine (42); (b) from asparagine (10) to aspartic acid (45); and (c) from asparagine (10) to threonine (49) is missing.

Human prourokinase mutants

A DNA sequence of the yeast glyceraldehyde-3-phosphate dehydrogenase (GAP-DH) promoter and a process for preparing a heterologous protein utilizing said DNA sequence as a promoter are disclosed, said GAP-DH promoter comprising a region upstream of an initiator codon of the GAP-DH protein up to -164 bp as a minimum unit. Since the GAP-DH promoter is small in size, a physiologically active substance can be expressed in yeasts effectively and recombination of DNA can be simplified.

Yeast promoter truncated GAP-DH

A method of producing a heterologous protein in yeast is disclosed, which comprises transforming a yeast Saccharomyces cerevisiae with recombinant DNA comprising a promoter selected from the group consisting of a yeast promoter or a hybrid promoter derived from a yeast promoter, particularly a hybrid promoter containing the enhancer region of SV40 virus, and a gene coding for a heterologous protein such as HBsAg and Pre S-HBsAg, particularly full length Pre S-HBsAg culturing, the resulting transformed cells to express the gene coding the heterologous protein, and isolating the heterologous protein from the cultured medium. GAP-DH and PH05 promoters and miniaturized ones can be used as the yeast promoter. The expression of the full length Pre S-HBsAg is carried out in the same expression system as that for 2nd or 3rd Pre S-ABsAg.

Yeast promoter and process for preparing heterologous protein

A recombinant plasmid wherein at least two DNA fragments containing a Baker gene and a Charlie gene of the yeast 2 .mu.m circular plasmid are incorporated into a plasmid containing genes encoding a physiologically active substance is disclosed. Also, a strain transformed by the recombinant plasmid is disclosed.

Recombinant plasmid and microbial strain transformed by said plasmid

A process for the purification of HBsAg is disclosed, which comprises adsorbing specifically on a carrier, in the presence of an inorganic salt in an amount of 5 to 25 W/V %, an HBsAg obtained by gene engineering.

Process for the purification of HBsAg

A live mumps vaccine can be stabilized to a great extent by adding in an amount, effective for stabilizing said vaccine, at least one stabilizer selected from the group consisting of albumin and gelatin.

Live mumps vaccine and method of stabilizing the same

Process for recovering interferon

A process for recovering interferon which comprises contacting a solution containing an interferon produced by the induced cells of human origin with a water-insolubilized sulfated polysaccharide to allow the interferon to be adsorbed on the water-insolubilized sulfated polysaccharide and then selectively eluting the interferon with an aqueous solution of an inorganic salt.

Double-stranded RNA synthesized using native human DNA as template is found to be an excellent interferon inducer with low toxicity in spite of homologous native to host cell. It is produced by reacting ATP, GTP, CTP and UTP with one another in the presence of a native human DNA as template by the catalytic action of an active RNA polymerase to form RNA and subjecting the resulting RNA to annealing by heating it at a temperature of 70.degree. to 100.degree. C. and gradually cooling to room temperature or below to form double-stranded regions between their molecules.

Interferon inducer and method of preparing same

Growing community of inventors

Osaka, Japan

Hirofumi Arimura