Novozymes A/s

Novo Zymes North America, Inc

Novozymes Als

Other

Novozymes, Inc.

Novozyms A/s

Novezymes Noth America, Inc

Novozymec North America Inc.

Hiroaki Udagawa

Shiro Fukuyama

Eric Allain

Tomoko Matsui

Chee Leong Soong

Rikako Taira

Ye Liu

Junxin Duan

Lene Nonboe Andersen

Wenping Wu

Shinobu Takagi

Anders Vikso Nielsen

Sara Maria Landvik

Hiroshi Teramoto

Markus Sakari Kauppinen

Chee-Leong Soong

Jiyin Liu

Torben Peter Frandsen

Jan Lehmbeck

Carsten Mailand Hjort

Michiko Ihara

Tom Anton Busk Nielsen

Suchindra Maiyuran

Howard Brody

Anders Viksoe-Nielsen

Christian Isak Jorgensen

Dominique Aubert Skovlund

Anders Vikso-Nielsen

Qiming Jin

Michael Lynge Nielsen

Soren Christensen

Christian Isak Jørgensen

S&oslash;ren Christensen

S Ren Christensen

Carsten Hjort

Shinobu Tkagi

Carsten M Hjort

Eric Room 103 Er Dan Yuan In Building 3 Allain

Sara Landvik

Chee-Leong Soon

Seiichiro Kishishita

The present invention relates to filamentous fungal cells secreting a polypeptide of interest, wherein the expression of a citT gene is altered, reduced or eliminated compared to a non-mutated otherwise isogenic or parent cell, and methods of producing a secreted polypeptide of interest in said cells as well as methods of producing said cells.

Modified filamentous fungal host cell for encoding a secreted polypetide of interest

The present invention relates to a recombinant fungal host cell comprising at least one first polynucleotide encoding a polypeptide of interest; and one or more second polynucleotide encoding a fungal PepC protease, wherein the one or more second polynucleotide is operably linked to a regulated heterologous promoter, as well as a method for producing a polypeptide of interest, comprising cultivating said fungal host cell.

Regulated PepC expression

The invention relates to a method for the simultaneous integration of two or more copies of a polynucleotide of interest into the chromosome of a fungal host cell comprising at least two pairs of recognition sequences of a site-specific recombinase, each pair flanking a resident negative selection marker; transformation of the cell with a construct carrying a gene of interest also flanked by the recognition sequences to ensure double-crossover events after transient expression of the recombinase, followed by selection for excision of all negative selection markers from the cell.

Simultaneous site-specific integrations of multiple gene-copies

The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.

Enzymes for starch processing

The present invention relates to isolated polynucleotides having leader sequence function. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods using the polynucleotides for production of polypeptides.

Polynucleotides having leader sequence function

Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi

The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

Methods for recombinant expression of a polypeptide comprising a carbohydrate binding module using a host cell having reduced expression of a protease

The present invention relates to isolated polynucleotides having promoter activity the use of the isolated polynucleotides for the production of a polypeptide. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing a desired polypeptide using the polypeptide having promoter activity.

Polynucleotides having promoter activity

The present invention relates to isolated polynucleotides having promoter activity the juse of the isolated polynucleotides for the procuction of a polypeptide. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing a desired polypeptide using the polypeptide having promoter activity.

The present invention relates to methods for reducing or eliminating the expression of a target gene in a filamentous fungal strain, comprising: (a) inserting into the genome of the filamentous fungal strain a double-stranded transcribable nucleic acid construct comprising a first nucleotide sequence comprising a promoter operably linked to a homologous coding region of the target gene and a second nucleotide sequence comprising the homologous coding region, or a portion thereof, of the target gene, wherein the first and second nucleotide sequences are complementary to each other and the second nucleotide sequence is in reverse orientation relative to the first nucleotide sequence; and (b) inducing production of an interfering RNA encoded by the double-stranded transcribable nucleic acid construct by cultivating the filamentous fungal strain under conditions conducive for production of the interfering RNA; wherein the interfering RNA interacts with RNA transcripts of the target gene to reduce or eliminate expression of the target gene. The present invention also relates to the filamentous fungal strains and to methods of producing a biological substance of interest in such filamentous fungal strains.

Methods for eliminating or reducing the expression of a genes in a filamentous fungal strains

The present invention relates to an isolated polypeptide having PepC inhibitory activity as well as to a method for producing a heterologous polypeptide of interest in anhost cell comprising: (a) cultivating thehost cell comprising a first and a second nucleic acid sequences under conditions conducive for the expression of the polypeptides encoded by the said first and second nucleic acid sequences, and wherein the first nucleic acid sequence encodes a heterologous polypeptide of interest and the second nucleic acid encodes the inhibitor polypeptide of the invention, and wherein the inhibitor polypeptide is expressed from a recombinant nucleic acid construct resulting in an increased level of the inhibitor polypeptide compared to anhost cell not comprising the recombinant nucleic acid construct; and (b) recovering the heterologous polypeptide.

Fungal PepC inhibitor

Growing community of inventors

Yokohama, Japan

Hiroaki Udagawa