Takara Bio Inc.

Takara Shuzo Co., Inc.

Other

Hiroaki Sagawa

Ikunoshin Kato

Tatsuji Enoki

Nobuto Koyama

Eiji Kobayashi

Jun Tomono

Katsushige Ikai

Takeshi Sakai

Eiji Nishiyama

Hiromu Ohnogi

Takanari Tominaga

Harumi Ueno

Mitsuko Ideno

Hua-Kang Wu

Nobuko Muraki

Yoshiko Nomura

Michio Hagiya

Fu-Gong Yu

Takahiro Marui

Suzu Deguchi

Kiyozo Asada

Takashi Uemori

Hiroyuki Mukai

Yoshimi Sato

Hideto Chono

Kazue Miyake

Osamu Takeda

Atsushi Oshima

Kinuko Nagamine

Akiko Kato

Masahiro Shiraga

Tuo-Ping Li

Katsumi Sugiyama

Kazutoh Takesako

Teruya Nakamura

Junko Yamamoto

Hirokazu Kotani

Akihiro Kondo

Nobutsugu Hiraoka

Katsuhiko Yamamoto

Fuyuko Takashima

Tomoko Fujita

Shigetoshi Mizutani

Kazuyori Ochiai

Hitoshi Sakakibara

Tatsui Enoki

Masayuni Kishimoto

Haruo Oyashiki

Mariko Moriyama

Haruhisa Sawaragi

Yoko Tatsumi

Mariko Okawa

Kaori Akiyama

Motoko Ueda

Kinuko Ogawa

Shinji Okuda

Masayuki Kishimoto

The present invention relates to a method for preparing a cytotoxic lymphocyte characterized in that the method comprises the step of carrying out at least one of induction, maintenance and expansion of a cytotoxic lymphocyte in the presence of fibronectin, a fragment thereof or a mixture thereof.

Process for producing cytotoxic lymphocyte

The present invention provides a method for preparing a cytotoxic lymphocyte characterized in that the method comprises the step of carrying out at least one step selected from induction, maintenance and expansion of a cytotoxic lymphocyte using a medium containing serum and plasma at a total concentration of 0% by volume or more and less than 5% by volume, in the presence of fibronectin, a fragment thereof or a mixture thereof.

Process for producing cytotoxic lymphocytes

A method for transferring a gene into a fat cell or progenitor fat cell comprising the step of infecting the fat cell or progenitor cell with a retrovirus vector having a foreign gene in the presence of a substance having both of a retrovirus-binding site and a target cell-binding site in the molecule or a mixture of a substance having a retrovirus-binding site and a substance having a target cell-binding site, the target cell-binding site having a region that can bind to VLA-5 and/or a region that can bind to VLA-4.

Method for transfer of gene into fat cell or progenitor fat cell

A method for preparing lymphocytes characterized in that the method comprises the step of carrying out expansion in the presence of (a) fibronectin, a fragment thereof or a mixture thereof, (b) a CD3 ligand, and (c) a CD28 ligand.

Method of producing lymphocytes

A method of producing lymphocytes characterized by comprising the step of culturing lymphocytes in the presence of a modified recombinant fibronectin fragment which has overlapping parts of the heparin-binding domain of fibronectin. This method makes it possible to achieve a high cell proliferation rate. The lymphocytes obtained thereby are appropriately usable in, for example, adoptive immunotherapy and, therefore, expected as highly useful in the clinical field. Moreover, a novel modified recombinant fibronectin fragment is provided.

The present invention is a method for inducing cytotoxic T cell having an antigen-specific cytotoxic activity, a method for maintaining the cell, a method for continuously culturing the cell or a method for expanding the cell, comprising the step of culturing a cytotoxic T cell in the presence of at least one substance selected from the group consisting of (A) a substance having a binding activity to CD44; (B) a substance capable of regulating a signal emitted by binding a CD44 ligand to CD44; (C) a substance capable of inhibiting binding of a growth factor to a growth factor receptor; (D) a substance capable of regulating a signal emitted by binding of a growth factor to a growth factor receptor; and (E) fibronectin, a fragment thereof or a mixture thereof.

Method of extended culture for antigen-specific cytotoxic lymphocytes

The present invention provides methods for inducing, maintaining and expanding CTL (cytotoxic T cell) having an antigen-specific cytotoxic activity at a high level, which is useful in the adoptive immunotherapy, by using as an effective ingredient at least one compound selected from the group consisting of acidic polysaccharides, acidic oligosaccharides, acidic monosaccharides, and salts thereof. The above-mentioned compounds include fucoidans, heparins, alginic acid, chondroitin sulfate A, chondroitin sulfate B, pectic acid, hyaluronic acid, degradation products of fucoidans, sulfated glucose, sulfated fucose and salts thereof.

Method of extensive culture of antigen-specific cytotoxic T cells

To find a substance which has a skin cosmeticizing effect such as prevention of skin aging, amelioriation of sensitive skin, or antipruritic action, and can serve as an effective ingredient for a hair-care product, thereby providing cosmetics comprising the effective ingredient. Concretely, there are provided cosmetics characterized in that the cosmetics comprise as an effective ingredient a compound selected from a fucoidan, a degradation product thereof, a sulfated monosaccharide or a salt thereof.

Fucoidan-containing cosmetics

Production of efficiently producing agaro-oligosaccharide; a polypeptide having an agarase activity which is usable in, for example, efficiently extracting a substance such as a nucleic acid from an agarose gel; the amino acid sequence of the polypeptide; a gene encoding the polypeptide; a process for producing the polypeptide; a process for producing agaro-oligosaccharide; and a process for extracting a substance such as a nucleic acid from an agarose gel.

Agarase and gene thereof

A polypeptide having an &#x3b1;-agarase activity, a gene encoding the polypeptide, a method for producing the polypeptide by genetic engineering and a method for producing an agarooligosaccharide using the polypeptide.

&#x3b1;-agarase and process for producing the same

The present invention provides a novel chalcone compound, its derivative or a salt thereof, each having a suppressive action of NO production or an inhibitory action of aldose reductase. In addition, the present invention provides a medicament, a food, a beverage or a feed having a therapeutic or prophylactic effect for a disease showing sensitivity to the compound, by utilizing the physiological actions of the compound.

Chalcone compounds

Medicinal compositions for treating, ameliorating or preventing diseases with sensitivity to 3,6-anhydrogalactopyranose represented by formula (1): foods, drinks, cosmetics, etc. containing as the active ingredient at least one member selected from the group consisting of the above-mentioned compound, its aldehyde, its hydrate and 2-O-methylated derivatives thereof and soluble sugar compounds containing the above compound. This compound also shows, for example, an apoptosis-inducing activity, a carcinostatic activity and inhibitory activities on the production of active oxygen, lipid peroxide radicals and NO, which makes it useful also as the active ingredient of antioxidants and preservatives.

Use of algae-derived physiologically active substances for treating a carcinomatous disease

A gene encoding a polypeptide having an activity of digesting sulfated fucogalactan which is useful in sugar chain engineering reagents, analyzing the structures of sulfated fucose-containing polysaccharides and preparing degraded products of the polysaccharides; a genetic engineering process for producing the polypeptide; and the polypeptide obtained by the process.

Sulfated fucogalactan digesting enzyme gene

The present invention relates to a chalcone compound represented by the following general formula: wherein Rand Rare taken together with the carbon atom to which they are bound, respectively, to form a hydroxydimethyl hexane ring, or where Ris a hydroxyl group, Ris an isohexenyl group, a derivative thereof, or a salt thereof. Also, the present invention relates to a therapeutic agent or prophylactic agent for a disease requiring neuronal protection, suppression for nitrogen monoxide (NO) production, inhibition for aldose reductase or suppression for interleukin production, and a food, beverage or feed for treatment or prevention of the above-mentioned disease, characterized in that each comprises the above-mentioned compound as an effective ingredient.

Chalcone compound

Growing community of inventors

Kusatsu, Japan

Hiroaki Sagawa