Ventana Medical Systems, Inc.

Hiroaki Nitta

Thomas M Grogan

Jennifer Wong

Christopher Bieniarz

Jerome W Kosmeder, Ii

Michael Patrick Farrell

Brian Daniel Kelly

Phillip C Miller

Anthony L Hartman

Wenjun Zhang

Fabien Gaire

Catherine Wolf

Lidija Pestic-Dragovich

Mary Padilla

Angela Sattler

Jacques Cavadenti

Megan Lehrkamp

Richard Gniewek

Thomas Grogan

Jerome Kosmeder

The present invention provides a method and kit for detection of two or more target molecules in a single tissue sample, such as for gene and protein dual detection in a single tissue sample. Methods comprise treating a tissue sample with a first binding moiety that specifically binds a first target molecule. Methods further comprise treating the tissue sample with a solution containing a soluble electron-rich aromatic compound prior to or concomitantly with contacting the tissue sample with a hapten-labeled binding moiety and detecting a second target molecule. In one example, the first target molecule is a protein and the second is a nucleic acid sequence, the first target molecule being detected by immunohistochemistry and the second by in situ hybridization. The disclosed method reduces background due to non-specific binding of the hapten-labeled specific binding moiety to an insoluble electron rich compound deposited near the first target molecule.

Method for chromogenic detection of two or more target molecules in a single sample

The present disclosure provides methods of diagnosing cervical dysplasia and cervical cancer by detecting Na, K-ATPase expression. For example, it is demonstrated herein that expression of the Na, K-ATPase &#x3b2;1-subunit increases in cervical dysplasia and cervical cancer, relative to normal cervical tissue. Also provided are methods of treating cervical dysplasia and cervical cancer by decreasing the biological activity of the Na, K-ATPase. For example, therapeutically effective amounts of one or more Na, K-ATPase inhibitors can be applied to the cervix to treat cervical dysplasia and cervical cancer.

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The invention provides novel reagents, reagent kits, and methods for automated hybridization. More particularly, the invention provides reagents, reagent kits, and methods for automated in situ hybridization and automated hybridization on a microarray. The use of automated instruments for in situ hybridization and microarray hybridization dramatically reduces the amount of labor and time involved and also facilitates standardization of protocols and consistency between results.

Reagents and methods for automated hybridization

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Oro Valley, AZ, United States of America

Hiroaki Nitta