Kyushu University

Takara Bio Inc.

Hiroaki Matsukawa

Yoshizumi Ishino

Takeshi Yamagami

Takashi Uemori

Takehiro Sagara

Provided are various novel DNA polymerases. Provided are: a DNA polymerase comprising: an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 12, which has a substitution of arginine at position 651 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid; and a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 14, which has a substitution of proline at position 653 by an amino acid residue having a negatively charged side chain, preferably by asparatic acid or glutamic acid, more preferably by glutamic acid.

DNA polymerase

Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids '-A-A-A-A-A-A-A-A-A-' between the amino acid residue at position 736 and the amino acid residue at position 737, wherein: 

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Fukuoka, Japan

Hiroaki Matsukawa