Other

Hakushinkouseikai Foundation

Life Science Research Institute Ltd.

Kawasaki, Hiroaki

Abe, Hiroyuki

Hiroaki Kawasaki

Hiroyuki Abe

Yoshio Ebihara

The present invention relates to a method by which cells usable for an immune cell therapy are separated from peripheral blood and grown. The present invention makes it possible to provide immune system cells which are large enough in number to be used in the immune cell therapy.

Method for obtaining monocytes or NK cells

A method in accordance with the present invention includes the steps of: concentrating cord blood-derived mesenchymal stem cells; and causing the mesenchymal stem cells thus concentrated to grow with use of a particular factor while maintaining undifferentiated state of the mesenchymal stem cells.

Method for undifferentiated growth of mesenchymal stem cell and method for concentration of mesenchymal stem cell

The purpose of the present invention is to provide a means for proliferating a monocyte with high efficiency and in a simple manner. The present invention provides a proliferating agent for a monocyte, which consists of at least one component selected from Flt-3L, IL-3 and IFN-&#x3b3; and can be used before a treatment for differentiation of a monocyte into a dendritic cell. The present invention also provides a culture medium for use in the proliferation of a monocyte, which contains at least one component selected from Flt-3L, IL-3 and IFN-&#x3b3; and can be used before a treatment for differentiation of a monocyte into a dendritic cell. The culture medium for use in the proliferation of a monocyte according to the present invention may contain GM-CSF.

Proliferating agent for monocyte, culture medium for proliferating monocyte, method for producing monocyte, method for producing dendritic cell, and method for producing dendritic cell vaccine

At a boundary area between a high-pressure portion in which a discharge port of a fuel pump is disposed and a low-pressure portion in which an intake port is disposed, a pressure relief groove extending along the boundary area is formed so as to be communicated with the outside of the pump by pressure relief hole. Alternatively, a slit is formed communicated with the outside. Accordingly, a fuel at a high-pressure area portion can be drained with reliability. Further, by communicating the above pressure relief groove with a positioning pin insertion port, a single hole can serve both as a positioning pin insertion hole and a pressure relief hole.

Growing community of inventors

Tokyo, Japan

Hiroaki Kawasaki