National Research Council of Canada

Hiroaki Iwaki

Peter C K Lau

Yoshie Hasegawa

Cyclopentanone 1,2-monooxygenase (CPMO) from(previously) sp. strain NCIMB 9872 carries out the second step of a degradation pathway that allows the bacterium to use cyclopentanol as a sole carbon source for growth. In the present invention there is reported the localization of the CPMO-encoding gene (cpnB) on a 4.3-kb SphI fragment, the determination of its sequence. The 550-amino acid CPMO polypeptide (M, 62,111) encoded by the gene was found to have 36.5% identity with the sequence of cyclohexanone 1,2-monooxygenase (CHMO) ofsp. strain NCIMB 9871. The 62-kDa CPMO was expressed inas an IPTG-inducible protein.

Recombinant cyclopentanone monooxygenase [cpmo]

The invention relates to a new strain of(designated as HI-70) and to the isolation, cloning, and sequencing of a cyclododecanone monooxygenase-encoding gene (named cdnB) from said strain. The invention also relates to a new cyclododecanone monooxygenase and to a method of use of the cyclododecanone monooxygenase-encoding gene.

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Montreal, Canada

Hiroaki Iwaki