Ajinomoto Co., Ltd.

Hiroaki Fukada

Takayuki Asahara

Mitsunori Tokura

Masayuki Sugiki

Kenichi Hashiguchi

Uno Tagami

Kiyoshi Matsuno

Daiki Yahagi

Erika Yoshida

Hiroka Koguchi

A method for producing a protein is provided. An objective protein is produced by culturinghaving an objective protein-producing ability, which has been modified so that the activity of a YscB protein is reduced, in a culture medium.

Method for manufacturing protein

A method for producing an objective protein and a method for producing a disaccharide are provided. An objective protein is produced by culturingin a culture medium containing an expression inducer such as gentiobiose. A disaccharide is produced from a saccharide raw material by enzymatic conversion using a disaccharide synthesizing enzyme.

Method for producing a protein and disaccharide using a

5'-guanylic acid (GMP) is produced efficiently by allowing a microorganism to react with xanthylic acid (XMP), wherein said microorganism is able to convert xanthylic acid into 5&#x2032;-guanylic acid and has been modified so that the nagD gene does not function normally and 5&#x2032;-guanylic acid synthetase activity is enhanced.

Method for producing 5'-guanylic acid

By reacting inosinic acid (IMP) with a bacterium which has been modified so that IMP dehydrogenase activity and 5'-guanylic acid (GMP) synthetase activity are enhanced, GMP is produced.

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Kawasaki, Japan

Hiroaki Fukada