Osaka University

Shimadzu Corporation

Osaka Industrial Promotion Organization

Hiroaki Adachi

Tsuyoshi Inoue

Kazufumi Takano

Takatomo Sasaki

Yusuke Mori

Hiroyoshi Matsumura

Shigeru Sugiyama

Masashi Yoshimura

Norihiko Nishizawa

Kazuyoshi Itoh

Seizo Morita

Atsushi Nakagawa

Junichi Takagi

Kei Ohkubo

Masayuki Abe

Noriaki Oyabu

Masahiro Ota

Takefumi Doi

Haruyasu Asahara

Mihoko Hirao

Kenji Iwasaki

Satoshi Murakami

Mika Hirose

Yuusuke Mori

Shutaro Ishida

Murakami Satoshi

Ryota Murai

Naoyuki Miyazaki

Yoshinori Takahashi

Masateru Kurata

Hiroshi Masuhara

Hiroshi Yoshikawa

Youichiroh Hosokawa

Satoshi Nakayama

The present invention provides a method for producing a substance with a modified carbon allotrope surface that can suppress or prevent uneven distribution, uneven orientation, and the like of a structural analysis target substance in a structural analysis by cryo-electron microscopy. A method for producing a substance with a modified carbon allotrope surface of the present invention includes: the step of surface-treating by reacting a carbon allotrope surface with a halogen oxide radical, wherein the carbon allotrope surface is modified by the surface-treating.

Method for producing substance with modified carbon allotrope surface, method for producing substance with carbon allotrope surface into which functional group is introduced, method for producing grid for cryo-electron microscopy, organic substance, and grid for cryo-electron microscopy

The present invention provides a target substance transfer method, a crystal production method, a composition production method, and a target substance transfer device, which allow the concentration of a target substance to be increased easily and effectively. The target substance transfer method is a method for transferring a target substancefrom a first phasethat is a liquid or solid phase containing the target substanceto a second phaseincluding: a phase approximation step of bringing the first phaseand the second phaseinto close proximity; and a bubble collapse step of forming bubbles in the vicinity of a boundary between the first phaseand the second phaseand then causing the bubbles to collapse.

Target substance transfer method, crystal production method, composition production method, and target substance transfer device

Provided are a method for observing protein crystal, wherein the growth process of the protein crystals is nondestructively and three-dimensionally monitored on a real-time basis and the growth of the crystals is controlled at a high accuracy to thereby enable the formation of single crystals having good qualities, which comprises observing the protein crystals, said protein crystals having been produced by a crystallization method using a gel, by an OCT measurement using light emitted from an ultrawideband light source; a method for observing protein crystals wherein the ultrawideband light source is an ultrawideband supercontinuum light source; a method for observing protein crystals wherein the center wavelength of the light emitted from the ultrawideband supercontinuum light source is a 0.8 &#x3bc;m band; and a method for observing protein crystals wherein the monitoring of the protein crystals is a monitoring by an in situ measurement.

Method for observing protein crystal

A method for analyzing a sample in a liquid is provided, which is suitable for easily and reliably preventing a liquid for analysis from being evaporated. When the sample in the liquid is observed by using a scanning probe microscope (SPM), a sealing liquid () immiscible with a liquid for analysis () is filled around the liquid for analysis (), in which a sample () and a probe () are immersed, so as to form a sealing state, in which the liquid for analysis () is isolated from an external gas. The SPM enables the probe () disposed on a front end of a cantilever () to approach a surface of the sample () immersed in the liquid, scans the surface of the sample, and detects an interaction between the sample () and the probe (), thereby generating an image.

Method for analyzing sample in liquid

The present invention relates to a process for producing high-quality crystals of protein or organic substances easily and efficiently. A solution of protein or an organic substance is prepared and then is cooled slowly to be supersaturated to a low degree. This supersaturated solution is irradiated with a femtosecond laser. A local explosion phenomenon occurs at the focal point of the laser and thereby a crystalline nucleus is generated. A high-quality crystal is obtained when a crystal is grown on the crystalline nucleus over a long period of time. The femtosecond laser to be used herein can be a titanium:sapphire laser having a wavelength of 800 nm, a duration of 120 fs, a frequency of 1 kHz, and an output of 400 mW.

Process for producing crystalline nucleus and method of screening crystallization conditions

Growing community of inventors

Suita, Japan

Hiroaki Adachi