University of Calcutta

Hirak Kumar Patra

Anjan Kr Dasgupta

Arka Mukhopadhyay

Krishanu Chakraborti

Dhrubajyoti Chattopadhyay

A method includes detecting DO in a sample by fluorescence spectroscopy. The fluorescence spectroscopy may be near-infrared fluorescence spectroscopy. The method may include observing an excitation wavelength of the sample at 620 nm to 640 nm. The method may also include observing an emission wavelength of the sample at 900 nm to 1000 nm. The method includes detecting DO and DO nano-clusters and their alterations in presence of suspended or colloidal objects including bio-molecules or cells, by emission spectroscopy.

NIR fluorescence of heavy water

Provided herein are compositions and methods for enhancing enzyme activity, half-life and/or thermostability. Also provided herein are compositions and methods including the enhanced enzymes. Also provided herein are methods and compositions related to improved pectinolytic enzymes, such as pectate lyase, which exhibit enhanced activity, thermostability and/or longer half-life.

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District West Midnapore, India

Hirak Kumar Patra