University of Alberta

Other

Allele Biotechnology & Pharmaceuticals, Inc.

Hiofan Hoi

Robert Earl Campbell

Jiwu Wang

Wei Zhang

Nathan Christopher Shaner

Sinoj Abraham

Jeffrey Germain

Kyle Minor

Carlo David Montemagno

Yuan He

Genetically encoded, photocleavable proteins are derived from a fluorescent protein. Upon illumination, the proteins photocleave and spontaneously dissociate into two or more fragments or release one end of an internal loop.

Genetically encoded photocleavable proteins

Constructs having membrane proteins stabilized by functionalized beta-sheet peptides are provided. The constructs can be associated with or covalently linked to supports. The support can be a membrane. The membrane can be used to selectively move desired particles from one side of the membrane to the other while impeding passage of undesired particles through the membrane. Methods of making and using such constructs and membranes are provided.

Functionalized beta-sheet peptide stabilized membrane proteins, constructs comprising same, and methods of forming and using same

The present disclosure provides an isolated nucleic acid sequence encoding a monomeric photoconvertible fluorescent protein, and fragments and derivatives thereof. Also provided is a method for engineering the nucleic acid sequence, a vector comprising the nucleic acid sequence, a host cell comprising the vector, and use of the vector in a method for expressing the nucleic acid sequence. The present disclosure further provides an isolated nucleic acid, or mimetic or complement thereof, that hybridizes under stringent conditions to the nucleic acid sequence. Additionally, the present provides a monomeric photoconvertible fluorescent protein encoded by the nucleic acid sequence, as well as derivatives, fragments, and homologues thereof. Also provided is an antibody that specifically binds to the photoconvertible fluorescent protein.

Photoconvertible fluorescent proteins

Growing community of inventors

Edmonton, Canada

Hiofan Hoi