Mjo Innovation Limited

Mjo Innovations Limited

Hilary Sian Whitworth

Ajit Lalvani

Graham Taylor

Katrina Mary Pollock

There is provided methods of determining tuberculosis (TB) infection status in an individual comprising: (i) providing a sample comprising T-cells; (ii) exposing the sample of (i) to one or more TB antigens; (iii) identifying T-cells in the sample that are CD4 positive and (a) secrete TNF-&#x3b1; without secreting IFN-&#x3b3;; or (b) secrete IFN-&#x3b3; without secreting TNF-&#x3b1;; (iv) identifying those cells of (iii) which are also CCR7 and, CD127 negative; and optionally (v) calculating the cells identified in (iv) as a percentage of those identified in (iii); wherein the identification of cells in (iv) and/or the percentage of T-cells calculated in (v) correlates to TB infection status of the individual, and wherein steps (iii) and (iv) can be carried out either sequentially or simultaneously. There are also provided compositions and kits for use in such methods.

Methods and kits for determining tuberculosis infection status

There is provided methods of determining(TB) infection status in an individual comprising: (i) providing a sample comprising T-cells; (ii) exposing the sample of (i) to one or more TB antigens; (iii) identifying T-cells in the sample that are CD4 positive and (a) secrete TNF-&#x3b1; without secreting IFN-&#x3b3;; or (b) secrete IFN-&#x3b3; without secreting TNF-&#x3b1;; (iv) identifying those cells of (iii) which are also CCR7 and, CD127 negative; and optionally (v) calculating the cells identified in (iv) as a percentage of those identified in (iii); wherein the identification of cells in (iv) and/or the percentage of T-cells calculated in (v) correlates to TB infection status of the individual, and wherein steps (iii) and (iv) can be carried out either sequentially or simultaneously. There are also provided compositions and kits for use in such methods.

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London, United Kingdom

Hilary Sian Whitworth