Hewlett-packard Development Company, L.P.

Hilary Ely

Matthew David Smith

Chantelle Domingue

Michael W Cumbie

Jeffrey Allen Nielsen

Manish Giri

Christie Dudenhoefer

Kenneth Ward

Joshua M Yu

Rachel M White

Erik D Torniainen

Diane R Hammerstad

Chien-Hua Chen

Alexander N Govyadinov

Jeremy Sells

Adam Higgins

Tod Woodford

Pavel Kornilovich

George H Corrigan

Silam J Choy

Brett E Dahlgren

Si-lam Choy

John Lahmann

Si-Lam J Choy

Daniel C Curthoys

A fluid thermal processing device may include a substrate, a platform projecting from the substrate, a fluid heating element supported by the platform, a temperature sensing element, distinct from the fluid heating element, supported by the platform and an enclosure supported by and cooperating with the substrate to form a fluid chamber about the platform. The fluid chamber forms a volume of uniform thickness conforming to and about the platform.

Fluid thermal processing

Examples herein involve amplification and detection of nucleic acids using a microfluidic reaction chamber. An example apparatus includes a reaction-chamber circuit to process a reagent and a biologic sample for amplification of nucleic acids. The apparatus further includes a plurality of capillaries to pass the reagent and the biologic sample through the microfluidic reaction chamber. A valve control system may selectively control each of a plurality of valves to cause the reagent and the biologic sample to selectively move through the microfluidic reaction chamber for the amplification of the nucleic acids according to a particular timing sequence. In various examples, a trapping region disposed in the microfluidic reaction chamber secures the nucleic acids in the microfluidic reaction chamber for amplification using the reaction-chamber circuit.

Microfluidic reaction chamber for amplification of nucleic acids

The present disclosure is drawn to microfluidic devices. A microfluidic device can include a substrate, a lid mounted to the substrate, and a microchip mounted to the substrate. The lid mounted to the substrate can form a discrete microfluidic chamber between structures including an interior surface of the lid and a portion of the substrate. The lid can include an inlet and a vent positioned relative to one another to facilitate loading of fluid to the discrete microfluidic chamber via capillary action. A portion of the microchip can be positioned within the discrete microfluidic chamber.

Microfluidic devices with lid for loading fluid

A microfluidic device includes a microfluidic chamber fluidly coupled to an inlet port and an outlet port, a semiconductor microchip including fluid active circuitry and transistor circuitry, and a bed of solid supports positioned within the microfluidic chamber fluidly positioned between the inlet port and the outlet port. The transistor circuitry in this example provides onboard logic at the semiconductor microchip to control fluid active circuitry. The semiconductor microchip also defines a portion of the microfluidic chamber.

Microfluidic devices

A fluidic device may include a vertical fluid dispensing volume having a side outlet, a fluid channel connected to the vertical fluid dispensing volume below the side outlet and a fluid actuator asymmetrically located between ends of the fluid channel to form an inertial pump to vertically pump fluid within the channel to the side outlet.

Inertial pump fluid dispensing

A method may include maintaining a sample comprising an ionic species and an optical indicator at an elevated temperature above 25&#xb0; C. on a semi-conductive microfluidic die during an incubation period, intermittently interrogating the sample with an interrogating light during the incubation period and sensing a response of the sample to the interrogating light, wherein the sample is interrogated with the interrogating light only during those times at which the sample is being sensed.

Ionic species interrogation and sensing

An apparatus includes a pipette dispenser to control dispensing of a volume to a dispensing location. A tip is operatively coupled to the pipette dispenser. The tip includes an electromechanical print head to dispense the volume from the pipette dispenser to the dispensing location based on a command from the pipette dispenser that indicates an amount of the volume to be dispensed from the print head.

Pipette dispenser tip utilizing print head

According to an example, a microfluidic apparatus may include a channel, a foyer, in which the foyer is in fluid communication with the channel and in which the channel has a smaller width than the foyer, a sensor to sense a property of a fluid passing through the channel, a nozzle in fluid communication with the foyer, and an actuator positioned in line with the nozzle. The microfluidic apparatus may also include a controller to determine whether the sensed property of the fluid meets a predetermined condition and to perform a predefined action in response to the sensed property of the fluid meeting the predetermined condition.

Microfluidic apparatuses

The present disclosure is drawn to microfluidic chips. The microfluidic chips can include an inflexible material having an elastic modulus of 0.1 gigapascals (GPa) to 450 GPa. A microfluidic channel can be formed within the inflexible material and can connect an inlet and an outlet. A working electrode can be associated with the microfluidic channel and can have a surface area of 1 &#x3bc;mto 60,000 &#x3bc;mwithin the microfluidic channel. A bubble support structure can also be formed within the microfluidic channel such that the working electrode is positioned to electrolytically generate a bubble that becomes associated with the bubble support structure.

Microfluidic chips

An apparatus includes a media that includes an encoded pattern to indicate a location of each of a plurality of dispensing locations on a receiving area for a pipette dispenser. The encoded pattern is employed to guide the pipette dispenser to dispense a volume to a selected dispensing location from the plurality of dispensing locations based on a predetermined dispensing location on the receiving area.

Apparatus with encoded media to indicate dispensing locations for pipette dispenser

According to an example, a microfluidic apparatus may include a fluid slot, a foyer in fluid communication with the fluid slot via a channel having a relatively smaller width than the foyer, a sensor to detect a presence of a particle of interest in a fluid passing through the channel, a nozzle in fluid communication with the foyer, and an actuator positioned in line with the nozzle. The microfluidic apparatus may also include a controller to receive information from the sensor, determine, from the received information, whether a particle of interest has passed through the channel, and control the actuator to expel fluid in the foyer through the nozzle based upon the determination.

Growing community of inventors

Corvallis, OR, United States of America

Hilary Ely