Takara Bio Inc.

Takara Shuzo Co., Inc.

Hikaru Takakura

Ikunoshin Kato

Kiyozo Asada

Mio Morishita

Tomoko Shimojo

Nobuto Koyama

Junichi Mineno

Masanori Mitta

Susumu Tsunasawa

Katsuhiko Yamamoto

Takashi Uemori

Tatsuji Enoki

Yoshimi Sato

Hirofumi Yoshioka

Kazuyori Ochiai

Hiroshi Kobori

Yasuhiro Kawano

Yuji Hashimoto

Ryo Hirano

Shuhei Sakamoto

Hiroyuki Mukai

Naoyuki Awazu

Masanari Kitagawa

Katsuyuki Dodo

Toshikazu Nishie

Toshihiro Shodai

Kazuhisa Shinmura

Kikyozo Asada

Ikunoshi Kato

Toshitake Okui

The present invention provides a method for efficiently manufacturing a non-enveloped virus with high purity without laborious operation by cultivating cells having the ability to produce a non-enveloped virus and bringing the cells and an acidic solution into contact with each other. A non-enveloped virus vector manufactured by the method of the present invention and a composition having the non-enveloped viral vector as an active ingredient are very useful as gene transfer methods in the fields of basic research and clinical application gene therapy.

Method for manufacturing non-enveloped virus

Provided are a production method for non-enveloped virus particles which is characterized in that a sample including non-envelopes virus particles is treated with PEG in at least two concentrations; a kit used in said production method; non-enveloped virus particles produced using said production method; and a pharmaceutical composition having the non-envelopes virus particles as an active ingredient.

Production method for non-enveloped virus particles

The present invention provides a method for producing a virus vector, which involves a step of culturing a cell capable of producing the virus vector in a culture medium containing a retinoic acid compound, a histone deacetylase-inhibiting substance and a substance capable of forming a chelate; and a culture medium for use in the production of a virus vector, which is characterized by containing a retinoic acid compound, a histone deacetylase-inhibiting substance and a substance capable of forming a chelate as active ingredients.

Method for producing viral vector

A polypeptide having an endonuclease activity derived from a psychrophilic microorganismsp. strain Ac10, which exhibits high activity at low temperatures, can remove any nucleic acid present in a protein solution and can reduce the viscosity of a protein extract; and a nucleic acid encoding the polypeptide.

Microorganism-derived psychrophilic endonuclease

Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides.

Thermotolerant ribonuclease H

Isolated DNAs which are DNAs having a base sequence represented by any of SEQ ID NOS:1 to 6 in Sequence Listing or fragments thereof and showing a stationary phase-specific promoter activity in gram positive bacteria.

Promoters

There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID Nos. 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.

Hyperthermostable protease gene

A hyperthermostable protease having the amino acid sequence represented by the SEQ ID NO:1 of the Sequence Listing or a sequence derived therefrom by deletion, substitution, insertion or addition of one to several amino acid residues, a gene encoding the hyperthermostable protease, and a process for preparing the protease, aiming at providing by genetic engineering techniques a hyperthermophile protease which is advantageous for industrial use.

Method for producing protease

Isolated DNAs which are DNAs having a base sequence represented by any of SEQ ID NOS: 1 to 6 in Sequence Listing or fragments thereof and showing a stationary phase-specific promoter activity in Gram-positive bacteria.

Promoters showing stationary phase-specific activity in gram-positive bacteria

Polypeptides having the amino acid sequence represented by SEQ ID NO:6 in Sequence Listing or an amino acid sequence derived from the above sequence by at least one of deletion, addition, insertion or substitution of one or more amino acids and showing a thermophilic glucoamylase activity.

Polypeptides having glucoamylase activity

There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID NOs: 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.

System for expressing hyperthermostable protein

Thermostable protease

Ultrathermostable protease genes

Growing community of inventors

Shiga, Japan

Hikaru Takakura