Lifesensors, Inc.

Abzyme Therapeutics LLC

Hiep Tran

Tauseef R Butt

Oxana A Malakhova

Steven D Weeks

Michael P Malakhov

Ian White

Christine Mary Prokopowitz

Xiaole Chen

Rolf Swoboda

Andrew F Phillips

Byung Dae Lee

Fouad Moussa

Carla Campbell

Rajesh Singh

Laura DeCristofano

Míchael P Malakhov

Salam Shaaban

Hung Pham

Hossein Askari

Michael Schwartz

The invention relates to new anti-hCD3 antibodies that, in contrast to prior art anti-CD3 antibodies, bind specifically to human CD3 at acidic pH, but do not significantly bind to human CD3 at neutral or physiological pH, methods to produce these antibodies and therapeutic uses of these antibodies. These antibodies are able to activate T cells at acidic pH while having significantly reduced activity at neutral or physiological pH.

Antibodies binding to human CD3 at acidic pH

The present invention provides a method for preparing a modular scaffold that can bind to a target antigen and a method for engineering a bispecific functional agent consisting of an existing polypeptide binder fused at its C-terminus with said modular scaffold.

Methods for generating bispecific functional agents

The present invention provides a triple-mode antibody display system that simultaneously matures, displays and secretes an antibody to a target of interest. An antibody in vivo-matured and complexed with membrane anchored bait can be expressed on the surface of the host cell, while complexed with a soluble bait the antibody is secreted from the host cell. Methods of using the system for identifying binders that bind specifically to an antigen of interest are also provided. Polypeptides, polynucleotides and host cells useful for making the protein binder display system are also provided along with methods of use thereof.

Triple-mode system for antibody maturation, surface display and secretion

Methods for enhancing expression levels and secretion of heterologous fusion proteins in a host cell are disclosed.

Methods and compositions for protein expression and purification

A yeast based transactivation assay for gender sorting is disclosed.

Compositions and methods for gender sorting

A yeast-based system and methods of use therefore are provided for identifying new molecules which activate nuclear receptors in a ligand-dependent fashion. In a preferred embodiment, a method is provided utilizing ecdysone receptor, USP and GRIP I encoding expression vectors which may be used to advantage for screening new and useful insecticidal compounds, detecting insecticidal residues as well as to regulate expression of a gene of interest in a host in a ligand-dependent manner.

Methods and compositions for identifying ligands for nuclear receptors

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West Chester, PA, United States of America

Hiep Tran