Ajinomoto Co., Ltd.

Hideyuki Suzuki

Takane Katayama

Hidehiko Kumagai

Shunichi Suzuki

Hiroyuki Nozaki

Isao Abe

Hidetsugu Nakazawa

Jun Takakura

Miyoko Nawata

Rie Takeshita

Takayuki Ito

Tatsuki Kashiwagi

Uno Tagami

Ayako Sasahara

Eri Tabuchi

A mutant glutamate-cysteine ligase (GSHA) suitable for generating &#x3b3;-Glu-Val, and a method for producing &#x3b3;-Glu-Val-Gly using the same are provided. &#x3b3;-Glu-Val is produced by using a mutant GSHA having a specific mutation with Glu and Val as raw materials, and &#x3b3;-Glu-Val-Gly is further produced by using &#x3b3;-Glu-Val and Gly as raw materials. &#x3b3;-Glu-Val-Gly is produced by using a mutant GSHA having a specific mutation with Glu, Val, and Gly as raw materials.

Mutant glutamate-cysteine ligase and method for manufacturing gamma glutamyl-valyl-glycine

A method for producing &#x3b3;-Glu-Val-Gly comprising the step of reacting Val-Gly with a &#x3b3;-glutamyl group donor in the presence of a &#x3b3;-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate &#x3b3;-Glu-Val-Gly, wherein the &#x3b3;-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.

Method for producing gamma-glutamylvalylglycine or a salt thereof

Method for producing &#x3b3;-glutamylvalylglycine or a salt thereof

A mutant tyrosine repressor that does not require tyrosine to induce expression of tyrosine phenol-lyase gene is obtained by introducing a mutation into a tyrosine repressor. A microorganism which is able to express large amounts of tyrosine phenol-lyase is obtained by introducing the mutant tyrosine repressor into the microorganism. The microorganism is useful for producing L-DOPA.

Mutant tyrosine repressor, a gene encoding the same, and a method for producing L-DOPA

A mutant tyrosine repressor that does not require tyrosine to induce expression of tyrosine phenol-lyase gene is obtained by introducing a mutation into a tyrosine repressor (tyrR), isolated from, or a tyrosine repressor (tyrR) which is encoded by SEQ ID NO. 1 and its derivative. A microorganism which is able to express large amounts of tyrosine phenol-lyase is obtained by introducing the mutant tyrosine repressor into the microorganism. The microorganism is useful for producing L-DOPA.

Mutant tyrosine repressor which does not require tyrosine to induce expression of the tyrosine phenol-lyase gene

A gene coding for a mutant tyrosine repressor having increased positive regulatory activity for expression of the tyrosine phenol lyase gene compared with a tyrosine repressor not having mutation is obtained by introducing a mutation into a tyrosine repressor gene of, introducing the gene into which the mutation is introduced intoexpressing a lactose operon under the control of a promoter and enhancer of the tyrosine phenol lyase gene derived from, and selecting a transformed strain having increased &#x3b2;-galactosidase activity.

Mutant tyrosine repressor proteins

Using as a host Escherichia coli which expresses lactose operon under the control of the promoter/enhancer of tyrosine phenol lyase gene derived from Erwinia herbicola, a DNA fragment coding for tyrosine repressor (tyrR) having an amino acid sequence depicted in SEQ ID NO: 2 in Sequence Listing is obtained from the chromosome gene library of Erwinia herbicola.

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Kyoto, Japan

Hideyuki Suzuki