Kaneka Corporation

Alpron Co., Ltd.

San-In Kensetsu Kogyo K.k.

Toda Biosystem Laboratory

Hideyuki Matsuda

Kazuyoshi Yajima

Makoto Kawamukai

Yasuhiro Ikenaka

Satomi Takahashi

Junzo Hasegawa

Youzi Omura

Kenichi Nishi

Masatoshi Sakamoto

Shuji Horie

Takashi Toda

Takumi Suizu

The present disclosure describes a method of producing a long-chain prenyl diphosphate synthase (in particular decaprenyl diphosphate synthase and solanesyl diphosphate synthase) using a gene and a protein which are required for enabling or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase as well as a method of efficiently producing a coenzyme Q having a long-chain isoprenoid in its side chain (in particular coenzyme Qor coenzyme Q) using a microorganism. The present disclosure also describes a DNA having a base sequence shown under SEQ ID NO:1, 3 or 5 and a DNA sequence derived from the above base sequence by deletion, addition, insertion and/or substitution of one to several bases thereof, and coding for a protein enabling (functioning) or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase in a cell of a host organism.

Method of expressing long-chain prenyl diphosphate synthase

It is an object of the invention to provide a process for easily producing a glucan, which assures a high degree of safety and which does not generate an oxidation off-flavor, without using a hydroxide of an alkali metal such as sodium hydroxide. In a process of the present invention, a physically pulverized yeast is autolyzed with an intracellular enzyme thereof, in an electrolyzed alkaline water which is obtained by electrolyzing water.

Process for producing yeast-derived glucan

The present invention provides a method of producing a long-chain prenyl diphosphate synthase (in particular decaprenyl diphosphate synthase and solanesyl diphosphate synthase) using a gene and a protein which are required for enabling or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase as well as a method of efficiently producing a coenzyme Q having a long-chain isoprenoid in its side chain (in particular coenzyme Qor coenzyme Q) using a microorganism. The present invention relates to a DNA having a base sequence shown under SEQ ID NO:1, 3 or 5 and a DNA sequence derived from the above base sequence by deletion, addition, insertion and/or substitution of one to several bases thereof, and coding for a protein enabling (functioning) or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase in a host microorganism.

The invention aims at providing a process for producing coenzyme Qefficiently in microorganisms by utilizing a coenzyme Qside chain synthesis gene derived from a fungal species belonging to the genusand genus The present invention relates to a DNA having a DNA sequence described under SEQ ID NO:1 and 2 or derived from the above sequence by deletion, addition, insertion and/or substitution of one or several bases and encoding a protein having decaprenyl diphosphate synthase activity.

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Process for producing coenzyme Q10

A gene of decaprenyl diphosphate synthase, which is the key gene participating in the biosynthesis of coenzyme Q was isolated from a bacterium belonging to the family Rhizobiaceae. By transferring this gene into a microorganism such as and expressing therein, coenzyme Q can be effectively produced.

Two strains of chitinolytic bacteria respectively named 'Corynebacterium Bine-A' 'Achromobacter Bine-B', which were deposited under deposit numbers FERM BP-2879 and FERM BP-2880 in the Fermentation Research Institute, Agency of Industrial Science and Technology, Japan. At least one of these strains is incubated on a chitin or chitosan medium, to prepare a culture material, which is usable directly as a solid antibacterial composition or fermented or aged to produce a solid antibacterial composition. Alternatively, water is added to the prepared culture material, and either of the liquid and solid phases may be used as an antibacterial, anti-nematode, and/or plant-cell activating composition.

Antibacterial, anti-nematode and/or plant-cell activating composition,

An anti-microbial composition or an anti-nematode agent including chitosan which is obtained by decomposing chitin with a strain of Enterobacter G-1, which was deposited in the Fermentation Research Institute, Agency of Industrial Science and Technology, Japan, or including a culture body which is obtained by cultivating the above strain of Enterobacter G-1 on a culture medium containing chitin.

Growing community of inventors

Matsue, Japan

Hideyuki Matsuda