Shimadzu Corporation

Wako Pure Chemical Industries, Inc.

Hideyoshi Arashima

Kenji Nakamura

Nobuhiro Hanafusa

Shinji Satomura

Koji Tanimizu

Masayoshi Hayashi

Tetsushi Okuyama

The object of the present invention is to provide a method for separating components in a liquid specimen and an apparatus used in said method, by which it is possible to avoid carryover even when the specimen injector is not washed using washing solution and also to avoid the increase of inner pressure in column of the separation device or ineffective separating operation even when many liquid specimens are separated by many times using the same separation device. In the present invention, the liquid specimen is introduced into the separation device from a direction opposite to eluting direction of the separation device which retains an adsorbent, and by separating and eluting the specimen from eluting outlet together with the eluant, trace quantity of insoluble substances and the specimen remained on the specimen introducing passage can be removed by elution procedure. As a result, it is possible to avoid carryover, to prevent the increase of inner pressure in the column of the separation device and to prevent ineffective separating operation caused by clogging of the column.

Method for separating components in liquid specimen and apparatus used

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Kyoto, Japan

Hideyoshi Arashima