Institut National De La Recherche Agronomique

Mitsubishi Kasei Corporation

Mitsubishi Corporation

Hideya Fujimoto

Takako Sakai

Takahiko Hayakawa

Ritsuko Yanagidate

Jun Imamura

Nobuya Koizuka

Ko Shimamoto

Kimiko Ito

Mikihiro Yamamoto

The object of the present invention is to isolate Rf gene, particularly Rf1 gene derived from radish, and identify its structure. The present invention provides a protein involved in restoration of a cytoplasmic male sterile individual to fertility which has 14 or more pentatricopeptide repeat (hereafter may be abbreviated to PPR) motifs, wherein a group of the motifs is divided into 3 or more blocks, each of the individual blocks has at least 2 or more PPR motifs, and the block in a carboxyl terminal (C terminal) side has 4 PPR motifs.

Protein involved in restoration of cytoplasmic male sterility to fertility and gene encoding the protein and gene

Protein involved in restoration of cytoplasmic male sterility to fertility and gene encoding the protein

A synthetic gene encoding an insecticidal protein, the gene is provided having a base sequence which has been modified to bring the codon usage in conformity with the genes of gramineous plants particularly rice plants. A preferred gene sequence is shown in SEQ ID NO: 1. An expression vector containing said structural gene is provided. A method for producing recombinant gramineous plants is provided, which comprises transforming protoplasts derived from gramineous plants with the vector, incubating the protoplasts in a liquid medium in the presence of cultured cells of the plants to form colonies and regenerating plants from said colonies. Recombinant gramineous plants having resistance against insects are also provided.

Synthetic insecticidal gene, plants of the genus oryza transformed with

Growing community of inventors

Tokyo, Japan

Hideya Fujimoto