Eiken Kagaku Kabushiki Kaisha

Toyo Seikan Kaisha, Ltd.

Toyo Seikan Group Holdings, Ltd.

Hidetoshi Kanda

Hiroaki Hayashi

Tsugunori Notomi

Toshihiro Yonekawa

Kentaro Nagamine

Shingo Saito

Tomoyuki Shibata

Yoshiaki Seto

Natsuki Masuya

Manabu Hosokawa

Yasuyoshi Mori

Yasuyuki Takeuchi

Yutaka Kubota

Satoru Michiyuki

Teijirou Kanada

Norimitsu Hosaka

Youichi Takano

Akira Onishi

The present invention addresses the issue of providing a target base sequence detection method, etc., whereby a determination can be readily made regarding whether or not a target base sequence is present in a nucleic acid sample. A fluorescent-labeled detection probe and a competitive probe are added to a nucleic acid sample and caused to hybridize with the nucleic acid in the sample, the fluorescence intensity is measured while changing the temperature of the reaction sample, and first order differentiation is performed on a temperature-fluorescence intensity curve. The fluorescent-labeled detection probe and competitive probe base length, base sequence, and amount to be added to nucleic acid samples are determined such that the first order derivative curve for a control target reaction sample including a target base sequence has a peak but the first order derivative curve for a control non-target reaction sample including a non-target base sequence does not substantially have a peak, when: the fluorescent-labeled detection probe and the competitive probe are added to both the control target nucleic acid sample and the control non-target nucleic acid sample; the fluorescence intensity is measured while the temperature of both the obtained control target reaction sample and the control non-target reaction sample are changed; and first order differentiation is performed on a temperature-fluorescence intensity curve.

Method for detecting a target base sequence

A compound container has a container main body forming a first accommodation chamber, an auxiliary container forming a second accommodation chamber, a mounting part on which the auxiliary container is mounted in the container main body, a cutting part which cuts a second accommodation chamber partition wall which partitions a part of the second accommodation chamber formed in the auxiliary container, a contents outlet port in the container main body, and an opening pouring element mounted on the contents outlet port and having another cutting part which cuts a first accommodation chamber partition wall which partitions a part of the first accommodation chamber formed in the container main body.

Compound container and pouring-out method

The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.

Method of nucleic acid amplification and measuring reagent and reagent kit therefor

Provided is a containerfor inspection which stores and pours out contents therein, and the container is provided with a container main bodyhaving a mouth portionformed thereon, a cover portionattached to the mouth portionto seal the container main body, and an opening capattached to the cover portion. The cover portionhas a closing wallfor closing the mouth portion, and the opening capis provided with an pouring outlet, and a cutting portionfor cutting the closing wall. When the opening capis attached to the cover portion, the cutting sectioncuts the closing wallto enable pouring out the contents. Thus, in the provided containerfor inspection, sealing properties of the container main bodyare ensured by the cover portion, and the closing wallof the cover portionis cut by the opening capattached to the cover portionwithout removing the cover portion, whereby the contents can be poured out.

Container for inspection

The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3'-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.

Method for detecting mutations and/or polymorphisms

Method of detecting variation or polymorphism

Growing community of inventors

Otawara, Japan

Hidetoshi Kanda