Dai Nippon Printing Co., Ltd.

Tokyo Medical and Dental University

Hideshi Hattori

Masatoshi Kuroda

Hideyuki Miyake

Ikuo Morita

Hironori Kobayashi

Daisuke Matsuura

Koji Eto

Makoto Nakamura

Masahiko Hase

Norihiko Okochi

Masayuki Ando

Hiroki Maeda

Masaaki Kurihara

Satoshi Narita

Satoshi Takeuchi

Akiko Kobayashi

Yoichi Takahashi

Tadafumi Shindo

Kazutoshi Awano

Yusuke Uno

Kei Ikegami

Tatsuya Tabei

Kazunari Ikeda

In accordance with the present invention, an implant in which cells are arranged in a fine pattern that is available for immediate implantation and that does not need to be removed after implantation is provided. The present invention relates to a cell-containing sheet, which comprises cells and a support comprising a bioabsorbable material, in which the support has a cell adhesion protein-containing layer on the surface thereof and the cells form a pattern on the support.

Cell-containing sheet

A main object of the present invention is to provide a cell transfer substrate capable of transplanting cells, maintaining a pattern as it is, on a living body tissue or the like, even when: the size of the cell sheet is extremely small; the cells are cultured sparsely; the cells are in a form of a small colony; or the cells are cultured in a pattern, for example, as a blood vessel, a vessel network such as a lymphatic vessel, or a nerve network, and to provide a substrate for cell transfer to be used for the cell transfer substrate. In order to achieve the above-mentioned object, the present invention provides a substrate for cell transfer comprising: a polymer base material; an intermediate layer formed on the polymer base material; and a cell transfer layer formed on the intermediate layer.

Substrate for cell transfer

An object of the present invention is to provide an artificial tissue construct that has means for transporting nutrients, oxygen, waste products, or the like and is viable in vivo. The present invention relates to a tissue construct formed in vitro, which comprises a vascular layer, a basal membrane layer, and a tissue-forming cell layer.

Artificial tissue construct and method for producing the same

An object of the present invention is to provide a means capable of transferring a cell sheet, a cell pattern or the like to a desired material at a high speed. The present invention provides a substrate for cell culture comprising a base and a cell adhesive region formed on a surface of the base, wherein the cell adhesive region is formed of a film that is rendered cell adhesive by applying an oxidation treatment and/or a decomposition treatment to a cell-adhesion inhibitory hydrophilic film containing an organic compound having a carbon-oxygen bond.

Substrate for cell culture

The present invention provides a method for performing a biological test under conditions in which an artificially prepared cell pattern with initial position coordinates that can be determined is three-dimensionally cultured within a gelled matrix. The present invention relates to a biological test method that comprises testing a biological indicator with reference to at least one selected from the group consisting of cell proliferation, cell movement, and cell differentiation in a cell pattern substantially embedded in gel. The present invention also relates to a kit for the biological test method.

Test kit comprising a culture instrument with a cell pattern and a gel suitable to embed cell pattern

It is an object of the present invention to provide a cell culture carrier for producing a cell sheet that can be readily detached from a cell culture carrier and is inhibited from contracting after being detached. The cell culture carrierof the present invention comprises: a support-held culture membranecomprising an organic thin filmhaving cell adhesion properties and biodegradability and a frame-like supportfixed on the periphery of the organic thin film for maintaining the dimensions of the organic thin film; and a base substratehaving a surfacewith a static water contact angle of 45&#xb0; or less, wherein the support-held culture membraneis detachably placed on the surfaceof the base substrate.

Cell sheet having good dimensional stability, method for production thereof, and cell culture carrier for use in the method

For the purpose of improving the accuracy of a variety analyses using a bio-microarray, it is a main object of the invention to provide a bio-microarray capable of producing relatively high signal intensity from a fluorescent molecule and capable of having improved quantification performance and to provide a substrate for bio-microarray. In order to achieve the object, the invention provides a substrate for bio-microarray characterized by having a reflection-suppressing function.

Bio-microarray and a substrate for use therewith

Test method using cells and test kit therefor

A main object of the invention is to provide a new method for producing a cell culture substrate used to cause cells to adhere in a highly precise form onto a base material and then culture the cells. To attain the object, the invention provides a method for producing a cell culture substrate comprising: a patterning substrate forming process of forming, on a base material, a cell culture patterning layer wherein a cell adhesion portion having cell adhesive properties and a cell adhesion-inhibiting portion having cell adhesion-inhibiting properties can be formed by action of a photocatalyst by energy irradiation, thereby forming a patterning substrate; an energy irradiating process of irradiating the energy onto the cell culture patterning layer, thereby forming the cell adhesion portion and the cell adhesion-inhibiting portion in a pattern form by action of the photocatalyst; and a cell-containing liquid applying process of applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method of applying the cell-containing liquid selectively onto the patterned cell adhesion portion.

Method for manufacturing cell culture substrate

A method for producing a cell culture substrate to which cells adhere in a finely processed pattern while retaining the pattern for a long period of time to culture the cells and a production apparatus used in the production method. The method includes: forming a patterning substrate by forming: on a base material, a light shielding portion, and a cell adhesive layer having adhesion to a cell and containing a cell adhesive material which is decomposed or denatured by an action of a photocatalyst upon energy irradiation so as to cover the light shielding portion; an energy irradiating process of irradiating energy to the patterning substrate from the base material side to form a pattern; and a cell adhesion process of making the cell adhere to the cell adhesion portion in a cell culture medium containing the cell and a culture medium.

Method for producing cell culture substrate and apparatus for producing cell culture substrate

Fine particles whose excitation light is not UV light or the like which has negative effects on a subject to be analyzed. The excitation light is emitted stably, and has excellent light emitting efficiency. Also a fluorescent probe including: fine particles containing a rare earth element excited by light having a wavelength in a range of 500 nm to 2000 nm and thereby emit up-conversion emission; and a specific binding substance which binds to the fine particles containing a rare earth element.

Fine particles containing rare earth element and fluorescent probe using the same

An objective of the present invention is to provide an antireflection film having a fine particle layer that is formed on a transparent substrate without allowing particles to aggregate as irregular lumps, while the region having no particles is being reduced to an area that is sufficiently small so as not to impair the visibility. In order to achieve the above-mentioned objective, the present invention provides an antireflection film that is characterized by having: a transparent substrate and a fine particle layer that is placed on the surface of the transparent substrate and made from at least a single layer of fine particles, wherein the surface of the transparent substrate and fine particles are allowed to adhere to each other by at least an electrostatic interaction, while the bulk of the above-mentioned fine particle layer is set to have a refractive index lower than the refractive index of the above-mentioned transparent substrate.

Growing community of inventors

Tokyo, Japan

Hideshi Hattori