Toyo Denka Kogyo Co., Ltd.

Hideo Takenaka

Yoshitaka Yamashita

Joji Takahashi

Tomio Kashiwai

Eiji Kanazawa

Masanobu Kamori

A carrier for immobilizing lipase is prepared by bonding a silane coupling agent having a carboxylic-ester group to an inorganic carrier. Preferred silane coupling agents are .gamma.-methacryl oxypropyltrimethoxy silane, .gamma.-acetoxypropyltrimethoxy silane or .gamma.-acryloxypropyltrimethoxy silane. The inorganic carrier can be kaolinite, porous glass, bentonite, silica gel, alumina, silica, silica-alumina hydroxy apatite or calcium phosphate gel. Lipase is immobilized by mixing lipase with the inorganic carrier containing the bound silane coupling agent, filtering the resultant mixture and drying the filtered mixture. Functional groups of the lipase are bonded to the carboxylic-ester group of the silane coupling agent.

Inorganic carrier containing bound silane coupling agent having

A method of preparing an enzyme immobilizing carrier includes the steps of providing a material consisting of kaolin mineral. The material is subject to acid treatment with a strong acid having a pH of 4 or less, and hydrothermal treatment at a temperature of from about 100.degree. C. to about 250.degree. C. The material is dried, thereby producing a porous powder. The porous powder is baked at a temperature of from about 350.degree. C. to about 1000.degree. C., for thereby producing the carrier.

Method of making a enzyme immobilizing carrier

An enzyme immobilizing carrier is formed from a porous powder. The powder uses kaolin mineral as a starting material, which starting material is subjected to acid treatment by a strong acid. The acid treated kaolin mineral is then subjected to a hydrothermal treatment. The acid and hydrothermal treated kaolin mineral is then subjected to a baking treatment.

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Kochi, Japan

Hideo Takenaka