Director-general of Agency of Industrial Science and Technology

Sumitomo Chemical Company, Limited

Other

Hideo Ohkawa

Yoshiyasu Yabusaki

Toshiyuki Sakaki

Megumi Shibata

Hiroko Murakami

Toshiyuki Sakai

Kenji Oeda

Masatoshi Shimizu

Yasunobu Ohkawa

Kenjirou Ozawa

Keiko Nakamura

Kazuyuki Oshie

Sakiko Hirose

Megumi Akiyoshi

Transgenic plants capable of metabolizing drugs are constructed by introducing a drug-metabolizing P450 molecular species into plants. These plants are capable of detoxifying and metabolizing foreign compounds such as environmental loads and extrinsic endocrine disruptors including drugs, poisons, and agrochemicals. Thus, they are highly useful when applied to field crops, etc. Also, a method for selecting the drug-metabolizing P450 molecular species is provided.

Plants capable of metabolizing drugs and use thereof

The expression of mitochondrial P450 in yeast is disclosed. The mitochondrial P450 includes a chimeric P450 in which a signal sequence present at the N-terminus of a mammalian mitochondrial P450 has been substituted by a signal of a yeast mitochondrial protein and a chimeric P450 in which the latter signal has been further substituted by a targeting signal sequence to microsome. An expression plasmid for producing the enzyme in a large scale, a recombinant yeast strain carrying the expression plasmid, a process for producing the enzyme by the use of the recombinant yeast, and a process for producing 5.beta.-cholestane-3.alpha., 7.alpha., 12.alpha., 27-tetrol, 25-hydroxyvitamin D.sub.3 and 1.alpha.,25-dihydroxyvitamin D.sub.3 are also disclosed.

Mitochondrial P450

The present invention provides a chimeric enzyme gene which codes for a monooxygenase having both monooxygenase activity derived from cytochrome P-450 and reducing power supplying ability derived from NADPH-cytochrome P-450 reductase. The present invention further provides a yeast expression plasmid which contains said chimeric enzyme gene and expresses said monooxygenase gene; a transformed yeast strain which carries said yeast expression plasmid; a monooxygenase which has both the monooxygenase activity and the reducing power supplying ability as mentioned above; and a process for producing said monooxygenase.

Chimeric fused monooxygenase of cytochrome P-450 and NADPH-cytochrome

Insecticidal protein of B. thuringiensis subsp. aizawai IPL is produced by growing a transformant microorganism harboring a recombinant expression vector carrying a gene coding for the insecticidal protein.

Production of insecticidal protein of bacillus thuringiensis subsp.

Transformed yeasts with yeast expression plasmid containing yeast alcohol dehydrogenase promoter and bovine adrenocortical cytochrome P-450.sub.17.alpha. cDNA, which produce bovine adrenocortical cytochrome P-450.sub.17.alpha., and processes for producing 17-hydroxypregnenolone and 17-hydroxyprogesterone which comprises 17-hydroxylating pregnenolone or progesterone to 17-hydroxypregnenolone or 17-hydroxyprogesterone, respectively, with the said transformed yeasts.

Transformed yeasts having steroid-hydroxylase activity and process for

Growing community of inventors

Hyogo, Japan

Hideo Ohkawa