Jnc Corporation

Kobe University

Mitsubishi Jidosha Kogyo Kabushiki Kaisha

Hideo Nishizawa

Noriyuki Ohnishi

Akihiko Kondo

Hirotaka Furukawa

Kageaki Matsui

Hideyuki Hata

Takako Nogami

The present invention provides a method for measuring oocyst of protozoa, such as, in an environment sample with high sensitivity at low cost within a short period of time; and a detecting reagent for use therein. Magnetic fine particles of 5 to 500 nm particle diameter having, immobilized thereto, binding factors for specific recognition of oocyst are added to an analyte containing a protozoan oocyst to form oocyst/binding factor/magnetic fine particle complexes by using a binding reaction to the oocyst, the formed complexes are recovered by a magnetic separation, and the protozoan oocysts contained in the complexes are assayed. Further, there is provided, for conducting the above method, a reagent for detecting protozoan oocysts comprising magnetic fine particles of 5 to 500 nm particle diameter having, immobilized thereto, antibodies against oocysts or binding factors for recognizing the antibodies.

Method for measuring protozoan oocyst and detecting reagent

Growing community of inventors

Tokyo, Japan

Hideo Nishizawa