Mie University

Hashimoto Electronic Industry Co., Ltd.

Hideo Miyake

Masaru Obata

Masatoshi Hashimoto

Yutaka Tamaru

Reiji Tanaka

Toshiyuki Shibata

Haruko Takeyama

Ryuji Uchida

Tetsushi Mori

Mano Hasegawa

Yoshihiro Murase

Mami Takahashi

Fumino Okuda

A method of treating a patient in need thereof includes administering a therapeutically effective amount of 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH) to serve or function as a nerve growth factor (NGF) activity promoter, preferably to treat and/or prevent a neurodegenerative disease, such as PolyQ disease, Parkinson's disease, Parkinson's syndrome, spinocerebellar degeneration, spastic paraplegia, amyotrophic lateral sclerosis, Alzheimer's disease, dementia with Lewy bodies or corticobasal degeneration.

Methods of treatment and prevention using 4-deoxy-L-erythro-5-hexoseulose uronic acid

Alginate lyase activity is exhibited by the amino acid sequences (polypeptides) shown in SEQ ID No:1 and SEQ ID No:2. These polypeptides and their homology equivalents are used to produce 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH) by contacting the polypeptide(s) with an alginate containing a uronic acid moiety and holding the mixture of the alginate and the polypeptide(s) at a temperature at which alginate lyase activity is exhibited.

DNA sequence and expression vector for alginate lyase

It is an object of the invention to provide a method and an apparatus for processing a very large number of fish eggs for gene injection and so on with a superior performance. An egg case has an egg-arranging plate and a cover plate. The fish eggs are dropped into concave portions of the egg-arranging plate, when the cover plate opens the concave portions. The cover plates closes the concave portions after fish eggs are accommodated in the concave portions. The egg case is fixed on a table of an egg-arranging apparatus. water is supplied on the egg-arranging plate in the egg plate. A scanning nozzle sprays water on upper surface of the egg-arranging plate. After closing the concave portions with the cover plate, the egg case is inclined for dropping fish eggs remaining on the cover plate.

Method and apparatus for processing a large number of fish eggs

It is an object of the invention to provide a method for processing a very large number of fish eggs for gene injection and so on, which employs a simple system with high performance and a reasonable construction expense. It is another object to provide the method having an water tank apparatus capable of producing a large number of fish eggs continuously. A large number of water tank groups having an independent drainage passage each is lighted up in turn with a predetermined interval. An egg-collecting case with a net bottom is set in the drainage passage connecting to the water tank group to which the lighting is started. The water tank group consists of a plurality of tank unit accommodating fishes each. Each book-shaped tank unit has a rectangular-shaped upper portion and a cone-shaped lower portion. Rotating water stream is formed in each tank unit.

Method for processing a large number of fish eggs

Growing community of inventors

Tsu, Japan

Hideo Miyake