Bristol-myers Company

Hideo Koshiyama

Hiroaki Ohkuma

Fumihide Sakai

The actinomycete 'Actinomadura sp.' designated in our culture collection as Strain No. G455-101 has been deposited with the American Type Culture Collection under the Accession Number ATCC No. 31491. The taxonomic description of this microorganism is presented in detail in the specification as are instructions for fermenting aqueous nutrient media with it to produce the BBM 928 antibiotic complex. The BBM 928 complex has utility as an antibacterial agent against gram-positive and acid fast bacteria and exhibits antitumor activity against experimental animal tumors.

Microorganism for BBM 928 production

BBM-928 is a mixture of antitumor antibacterial substances produced by fermentation of Actinomadura sp., ATCC No. 31491, under controlled conditions. The components may be separated by silica gel thin layer chromatography and by other means.

Fermentation process for BBM-928

An antitumor antibacterial complex referred to as BBM-928 is described which is produced by microbiological fermentation under controlled conditions using a species of actinomycetes. The bioactive components of the complex have pronounced antitumor activity and are moderately to weakly active against gram-positive and acid fast bacteria but practically inactive against gram-negative bacteria and fungi. Based on median survival time, the component designated BBM-928A is substantially more active than mitomycin C by a factor of 10 to 300 fold against intraperitoneally implanted mouse tumors.

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Musashino, Japan

Hideo Koshiyama