Osaka University

Hideo Goda

Isao Fuke

Michiaki Takahashi

Masahide Ishibashi

Toyokazu Ishikawa

Toshiyuki Onishi

Shinichi Miyake

Takeshi Nabeshima

Iwao Yoshida

Akihisa Takamizawa

Keisuke Takaku

Takeo Konobe

Kouichi Morita

Morita Kouichi

Toshiyuki Akiyama

A nucleic acid molecule containing nucleotide sequences that encode the capsid protein, pre-membrane protein and non-structural protein of Japanese encephalitis virus, and a nucleotide sequence that encodes the envelop protein of a second flavivirus, wherein the nucleotide sequence(s) that encode(s) the pre-membrane protein and/or non-structural protein of Japanese encephalitis virus contain(s) nucleotide mutations that produce one or more amino acid mutations that attenuate the virus.

Attenuated chimeric flavivirus bearing attenuated japanese encephalitis virus gene as backbone

Attenuated chimeric flavivirus bearing attenuated Japanese encephalitis virus gene as backbone

There is disclosed a method for purifying a gene-expression product produced by recombinant DNA technique which comprises a specific sequence of steps including adsorption treatment with silica gel, adsorption treatment with activated carbon, at least twice density gradient centrifugation and at least twice equilibrium density gradient centrifugation. The method of the present invention is very effective to remove allergen from gene-expression products contaminated therewith, enabling highly purified gene-expression products to be produced on a large scale.

Method for purifying a gene-expression product produced by recombinant

Growing community of inventors

Kanonji, Japan

Hideo Goda