Hitachi-high-technologies Corporation

Hidenori Namba

Jin Matsumura

Ryoji Inaba

Motohiro Yamazaki

Tomohiro Shoji

Kazumichi Imai

Seiichi Ugai

Hiromi Yamashita

Takashi Gomi

An electrophoretic apparatus that allows bubbles to be readily removed out of an electrophoretic passage. The passage for electrophoretic medium, at a connecting section where capillaries filled with electrophoretic medium are connected with a pumping mechanism for filling the electrophoretic medium, is arranged such that the side of the pumping mechanism is disposed below the side of the capillaries, so that the electrophoretic medium flows from down to up at the connecting section when filling the electrophoretic medium into the capillaries. Preferably, the passage between the capillary array and the buffer solution is controlled by using a rotary-type valve having high withstand pressure to simplify the passage structure. The dead volume of the passage can be reduced and the valuable electrophoretic medium can be efficiently used. The amount of used electrophoretic medium required for the removal of the bubble can be also reduced.

Electrophoretic apparatus

The present invention provides a capillary electrophoresis apparatus in which a capillary is easily attached to and detached from a migration medium filling unit without mixing impurities into the capillary. Mixture of impurities such as dust is also prevented when a capillary negative-electrode end is brought into contact with a sample stored in a vessel. Furthermore, temperature control is efficiently performed in the capillary. In the capillary electrophoresis apparatus, the whole of capillary array can be supported by grasping a grip portion by hand. A migration medium filling mechanism includes a slide mechanism which moves a polymer block with respect to a capillary head. The capillary electrophoresis apparatus includes a vessel in which a sample and a buffer can simultaneously be stored. Temperatures of the capillary and an irradiation and detection unit are controlled by a temperature control function provided in a thermostatic device.

Capillary electrophoresis apparatus

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Naka, Japan

Hidenori Namba