Matsushita Electric Industrial Co., Ltd.

Panasonic Corporation

Panasonic Intellectual Property Management Co., Ltd.

Hidenobu Yaku

Tetsuo Yukimasa

Hiroaki Oka

Daisuke Miyoshi

Hirokazu Sugihara

Nobuhiko Ozaki

Yukari Hataoka

Maki Yotsuhashi

Mari Onodera

Kimi Fukahi

The present disclosure provides a measurement method of telomerase activity with no occurrence of false-negative and high quantitative capability. In the measurement method of telomerase activity of the present disclosure, a solution containing telomerase as a sample solution is mixed with a solution containing primer DNA as a substrate for telomerase even as or after a solution containing non-primer DNA not as a substrate for telomerase is mixed therewith. Non-primer DNA can remove an effect of DNase in the sample solution and prevent occurrence of false-negative. Subsequently, the primer DNA is extended with the telomerase and the extended primer DNA is detected, through which telomerase activity is measured.

Method of extending DNA with telomerase and method of measuring telomerase activity

The present invention provides a method for determining whether or not an aqueous solution contains two or more cancer cells. The present method is characterized by the following three matters. First, the PCR solution contains the TS primer at a concentration of not less than 0.1 &#x3bc;M and not more than 1 &#x3bc;M in the present invention. Second, the PCR solution contains an ACX reverse primer. Third, the PCR solution contains the ACX reverse primer at a concentration of not less than 0.02 &#x3bc;M and not more than 0.06 &#x3bc;M in the present invention. In the present method, it is determined that an aqueous solution contains cancer cells even if the aqueous solution contains only two cancer cells.

Method for determining whether or not aqueous solution contains cancer cells by using improved telomerase assay

The present invention provides a method for determining whether or not a capillary filled with an electrophoretic medium is suitably used for electrophoresis before electrophoresis is performed using the analytes. The method comprises (a) applying an alternating-current voltage between a first electrode which is in contact with a first electrolyte solution in which one end of the capillary is immersed and a second electrode which is in contact with a second electrolyte solution in which the other end of the capillary is immersed to measure an electric conductivity of the electrophoresis medium with which an inside of the capillary is filled; and (b) determining that the capillary filled with the electrophoresis medium fails to be used suitably for the electrophoresis, when the electric conductivity is more than 4.2 mS/cm.

Method for determining whether a capillary filled with an electrophoresis medium can be used suitably for electrophoresis

A method is provided for specifically detecting a G-quadruplex, and the like. The method is characterized by including the steps of preparing a solution including an anionic planar phthalocyanine and mixing the solution with a sample solution to obtain a liquid mixture. The solution includes an anionic planar phthalocyanine. The method also includes a step of measuring the absorbance at 640 to 740 nm of the obtained liquid mixture.

Method for detecting G-quadruplex, method for detecting G-quadruplex-forming DNA and method for determining telomerase activity

An object of the present invention is to provide a method for inhibiting a DNA extension reaction by telomerase. More specifically, the present invention relates to a method for inhibiting a DNA extension reaction by telomerase, the method being characterized by including the step of adding an anionic phthalocyanine to a solution containing telomerase, a DNA to be a substrate of a telomerase reaction, and dNTPs.

Method for inhibiting telomerase reaction using an anionic phthalocyanine compound

The present invention provides a chipthat enables an immunoassay with high accuracy and without requiring a high volatile reagent. The chipincludes a chip substrate, a reagent mixtureand potassium ferricyanidethat are immobilized leaving a space in between on the chip substrate. The reagent mixtureincludes at least one selected from NADP and NADPH, malate dehydrogenase, and a substrate of malate dehydrogenase. The chipfurther includes diaphorase, immobilized on the chip substrate, as a part of or a component other than the reagent mixture

Chip for electrochemical immunoassay

Immobilization of malate dehydrogenase on a substrate using a glycerol solution containing malate dehydrogenase is achieved through dropping a mixed solution obtained by adding at least one selected from malic acid and malate to the glycerol containing malate dehydrogenase on the substrate, and drying it thereon. It is preferable to prepare the mixed solution by adding the malate to the glycerol solution containing malate dehydrogenase. The malate is preferably at least one selected from potassium malate and sodium malate.

Method of immobilizing malate dehydrogenase on a substrate

A liquid delivery apparatushas a rotary substrate. The substrateformed with a fluid passageA for communicating a supply chamberA and target chamberA. An inlet end portionat which the fluid passageA is connected to the chamberA extends in a clockwise direction Rof rotational directions of the substrate. The inlet end portionholds a liquidin the chamberA by a capillary force. The rotation drive unitrotates the substrateso that an inertial force exceeding the capillary force and directed to the clockwise direction Racts on the liquidin the inlet end portion. Delivery behavior control of microfluid with a high degree of flexibility can be achieved.

Liquid delivery apparatus and liquid delivery method

An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3' side of a target SNP base is G, while a base adjacent with one base spaced apart is G. According to the present invention, the 3&#x2032; end base is designed to be the base corresponding to SNP; the second base from the 3&#x2032; end to be T or G; the third base from the 3&#x2032; end to be any one of T or G; and the base sequence of from the fourth from the 3&#x2032; end to the 5&#x2032; end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3&#x2032; side to a desired base.

Discrimination method of target base in DNA, and allele specific primer used in the method of the same

The present invention provides a blood processing apparatus that can introduce a metered quantity of a blood sample into a chamber and does not require an opening linked to the chamber to be closed after the introduction of the blood sample. An introducing chamber, an injection port, and an air port are formed in a rotatable platform of a blood processing apparatus. The injection port links the introducing chamber with the outside of the rotatable platform. A blood sample is introduced from the injection port into the introducing chamber. A blood coagulant is held on the port wall surface of the air port and/or around a portion of the surface of the rotatable platform where the air port is open.

Blood processing apparatus and blood introducing method

An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3' side of a target SNP base is G, while a base adjacent with one base spaced apart is C. According to the present invention, the 3&#x2032; end base is designed to be the base corresponding to SNP; the second base from the 3&#x2032; end to be T or G; the third base from the 3&#x2032; end to be any one of A, T or C; and the base sequence of from the fourth from the 3&#x2032; end to the 5&#x2032; end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3&#x2032; side to a desired base.

An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3' side of a target SNP base is G, while a base adjacent with one base spaced apart is T. According to the present invention, the 3&#x2032; end base is designed to be the base corresponding to SNP; the second base from the 3&#x2032; end to be T or G; the third base from the 3&#x2032; end to be G or C; and the base sequence of from the fourth from the 3&#x2032; end to the 5&#x2032; end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3&#x2032; side to a desired base.

An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3' side of a target SNP base is A, while a base adjacent with one base spaced apart is G. According to the present invention, the 3&#x2032; end base is designed to be the base corresponding to SNP; the second base from the 3&#x2032; end to be C; the third base from the 3&#x2032; end to be any one of A, T or G; and the base sequence of from the fourth from the 3&#x2032; end to the 5&#x2032; end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3&#x2032; side to a desired base.

Growing community of inventors

Osaka, Japan

Hidenobu Yaku