Hitachi High-tech Science Corporation

Other

Cellseed, Inc.

Hideko Kanazawa

Amersham Pharmacia Biotech K.k.

Kanazawa, Hideko

Teruo Okano

Kazunori Kataoka

Yasuhisa Sakurai

Akihiko Kikuchi

Katsutoshi Shimizu

Yoshikazu Matsushima

A two-dimensional liquid chromatographic analyzer, in which a temperature control part changes a temperature in a single-step manner and switches the temperature at a high speed. Also, the temperature control part has a holder around a separation column so that the temperature of the separation column can be changed to a preset temperature. A first separation column thereof changes elution time(s) of the objective component(s) by temperature modulation and the mobile phase of the first separation column is an aqueous mobile phase having a constant composition.

Two-dimensional liquid chromatographic analyzer

Two-dimensional liquid chromatographic analyzer and analytical method

The amount of drug consumption caused by a specific drug-metabolizing enzyme and/or the amount of the resulting metabolite(s) is measured by chromatography with an aqueous mobile phase under temperature control using a packing material whose surface is coated with a polymer having a hydration force varying in the temperature range of 0&#xb0; C. to 80&#xb0; C. This system enables proper evaluation of drug-metabolizing capacity through simple means without adversely affecting the environment.

High performance liquid chromatography with an aqueous mobile phase for analysis of drug and its metabolite

To provide chromatographic packings whereby biological components, etc. which cannot be separated by either ion-exchange chromatography or reversed phase chromatography employed alone can be efficiently separated without deteriorating their activities. Use is made of a packing which contains a charged copolymer and makes it possible to change the effective charge density on the surface of a stationary phase by a physical stimulus while fixing a mobile phase to an aqueous system.

Packing material for chromatography having novel characteristic and method for isolation of substance using the same

PTH-amino acids can be analyzed within a short time at a high sensitivity by chromatographically separating said PTH-amino acids with the use of a packing wherein the hydrophilic/hydrophobic balance on the stationary phase surface can be changed by an external signal while fixing the mobile phase to an aqueous system.

Method for separating PTH amino acids

Growing community of inventors

Sagamihara, Japan

Hideko Kanazawa