Mitsui Chemicals, Inc.

Hideki Umetani

Hironori Komatsu

Tomoyuki Ando

Hitoki Miyake

Nobuyuki Fukazawa

Takeshi Nakamura

Toshihiro Oikawa

Atsushi Sakuma

Hiroki Ishibashi

Yasushi Fukuiri

Toshifumi Yamaki

Kazuhiko Togashi

Teruyuki Nagata

Hideaki Oikawa

Hidetoshi Hayashi

Tsuyoshi Inoue

Tadashi Araki

Katsutoshi Tsuchiya

Kiyoshi Ito

Kouki Fukumura

Tamotsu Asano

Hiroshi Sonoda

Toshio Kitashima

Junko Naruse

Ichirou Ikeda

Tsuyoshi Yasutake

Junya Fujiwara

Ken'ichi Goto

Takashi Shimaoka

Hirokazu Awano

Kyoko Maruyama

Takahiro Yamauchi

A novel gluconate dehydratase derived fromand a gene encoding the gluconate dehydratase are provided. By reacting the gluconate dehydratase or a transformed cell containing the gene with an aldonic acid, the corresponding 2-keto-3-deoxyaldonic acid can be efficiently produced.

Gluconate dehydratase

A desired isomer is selectively prepared by phosphorolyzing and isomerizing an anomer mixture of a 1-phosphorylated saccharide derivative while crystallizing one of the isomers to displace the equilibrium. Furthermore, using the action of a nucleoside phosphorylase, a nucleoside is prepared from the 1-phosphorylated saccharide derivative obtained and a base with improved stereoselectivity and a higher yield. This process is an anomer-selective process for preparing a 1-phosphorylated saccharide derivative and a nucleoside.

Process for selectively producing 1-phosphorylated sugar derivative anomer and process for producing nucleoside

5-trifluoromethyl-2,4-bis(trimethylsilyloxy)pyrimidine is reacted with a 2-deoxy-&agr;-D-erythro-pentofuranosyl chloride derivative reacted in the absence or presence of a small amount of solvent to give a &bgr;-form of 1-(2&prime;-deoxy-&bgr;-D-erythro-pentofuranosyl)-5-trifluoromethyl uracil derivative with a high selectivity.

Process for the preparation of trifluorothymidine derivatives

Fluorination agent and preparation and use of same

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Chiba, Japan

Hideki Umetani