Asahi Glass Company, Limited

Agc Inc.

Kanazawa University

Hideki Tohda

Yuko Hama

Hiromichi Kumagai

Futoshi Hara

Shuichiro Kimura

Alimjan Idiris

Mayumi Kobayashi

Tetsuya Kotani

Alimujiang Yidirei

Kaoru Takegawa

Toshiyuki Sasagawa

Kyotaro Hirashima

Susumu Zen-In

Yasuko Fujita

Kiyokazu Nikaido

Hiroko Tsukamoto

Provided are: an expression vector for secreting a protein (Z) to be recovered or a fusion protein having the protein (Z) moiety therein; a method for producing a transformant using the expression vector; the transformant; and a method for producing a protein using the transformant. An expression vector comprising an expression cassette containing a structural gene sequence (y) encoding a protein (Y), a structural gene sequence (z) located downstream from the structural gene sequence (y) and encoding a protein (Z) that is a protein to be recovered, and a promoter sequence and a terminator sequence for expressing a fusion protein containing the protein (Y) moiety and the protein (Z) moiety, characterized in that the protein (Y) is a full-length protein of protein disulfide isomerase 1 (PDI1), a partial protein of PDI1, or a mutant protein of the full-length protein or the partial protein.

Expression vector and method for producing protein

Provided is a transformant ofmutant which can produce and collect &#x3b2;-glucosidase without requiring complicated separation steps, and a vector which is useful for transforming a yeast of the genus. The transformant of amutant of the present invention is a transformant of amutant which exhibits an increased Gsf activity and decreased or no pyruvyl transferase Pvg1 enzymatic activity, and is comprised of a structural gene sequence encoding a &#x3b2;-glucosidase derived from a filamentous fungus, and a promoter sequence and a terminator sequence for expressing the structural gene in a chromosome or as an extrachromosomal gene. Further, the present invention relates to a cloning vector which is characterized by comprising a hsp9 gene promoter or an ihc1 gene promoter of a yeast of the genus, and is useful for transforming a yeast of the genus, an expression vector, a transformant, etc.

[object Object]

The present invention relates to a transformant, containing a lactate dehydrogenase gene which is introduced intoas a host, in which a part of a gene cluster encoding a pyruvate decarboxylase in thehost is deleted or inactivated.

Transformant and process for production thereof, and process for production of lactic acid

The object is to provide a transformant which can produce a heterologous protein having a structurally controlled O-linked sugar chain having an O-Man-Gal disaccharide structure, a method for producing the transformant by usingas the host, and provide a host for producing the transformant and a method for producing an O-glycosylated heterologous protein. Anhost having no omh1 gene or an inactivated omh1 gene in its chromosomes for producing an O-glycosylated heterologous protein having an O-linked sugar chain having an O-Man-Gal disaccharide structure by expression of the heterologous protein by a genetic engineering technique and subsequent glycosylation of the expressed heterologous protein. A transformant from the host, a method for producing the transformant and a method for producing an O-glycosylated heterologous protein by using the transformant.

Host, transformant and method for producing the transformant and method for producing O-glycosylated heterologous protein

The efficiency of production of a heterologous protein by a transformant of a yeast host is improved. A method of constructing a host for expression of a foreign gene which comprises deleting or inactivating at least one gene selected from the protease-associated genes (especially, metalloprotease genes and serine protease genes) of, a host in which the above-mentioned gene is deleted or inactivated, a transformant obtained by introducing a foreign gene into the host and a method of producing a heterologous protein using the transformant. The protease-associated gene is at least one gene selected from the group consisting of psp3 (SPAC1006.01), is sxa2 (SPAC1296.03), ppp51 (SPAC22G7.01) and ppp52 (SPBC18A7.01) or at least two genes selected from the group consisting of metalloprotease genes and serine protease genes, especially, three genes psp3 (SPAC1006.01), isp6 (SPAC4A8.04) and ppp53 (SPAP14E8.04). A host in which four genes consisting of the above three genes and ppp16 (SPBC1711.12), or five genes consisting of the above four genes and ppp22 (SPBC14C8.03) are deleted or inactivated is preferred.

Yeast host, transformant and method for producing heterologous proteins

The object of the present invention is to provide an edible vaccine which is effective for human papilloma virus (HPV) type 16 and available in large amounts inexpensively. An edible human papilloma virus vaccine obtained by culturing a transformant of an avirulent fission yeast host, wherein the transformant carries a gene encoding an antigenic protein of human papilloma virus introduced therein and accumulates the expressed antigenic protein in it.

Human papillomavirus vaccine for oral administration

A universal method for chromosome modification (deletion of a desired DNA region) which does not require any special enzymes or sequences is provided. Further, a method for determining whether the DNA region to be deleted contains a gene essential for growth of the cell under certain culture conditions is provided by utilizing the chromosome modification method.

Chromosome manipulation method

To provide a heterologous protein expression system which enables the fission yeastas a host to produce of a heterologous protein which is almost impossible to express, or if possible, produced at low levels, under control of conventional promoters. The present invention provides an expression for use in the fission yeast, a transformant carrying the expression vector, a method for producing a heterologous protein using the transformant, in particular a method for producing a heterologous protein in which a heat shock protein gene promoter inis used to regulate gene expression by application of a specific form of stress, whereby the timing of the production of the desired heterologous protein can be controlled.

Expression vector, a transformant carrying the same and a method for producing heterologous protein

In a method of constructing a eukaryotic host microorganism for production of a heterologous protein encoded by a trangenically introduced gene, production efficiency of the heterologous protein by the transformant obtained by introducing the gene encoding the heterologous protein into the host is improved. Part or all of the genome unnecessary or detrimental to production of the heterologous protein by the transformant in culture is deleted or inactivated. The eukaryotic host microorganism is preferably. The part of the genome of the eukaryotic host microorganism to be deleted or inactivated is preferably a gene selected from the genes associated with energy metabolism and the genes associated with proteases, such as a pyruvate decarboxylase gene, a serine protease gene, an aminopeptidase gene and a carboxypeptidase gene.

Method of constructing host and method of producing heterologous protein

A method for transforming,which comprises integrating a vector into a chromosome of,through homologous recombination, wherein the vector has an expression cassette containing a heterologous protein structural gene and a promoter and a gene segment which induces homologous recombination of the chromosome and has lost a replication origin which functions in cells of an organism other than,required for construction of the vector.

Growing community of inventors

Tokyo, Japan

Hideki Tohda