Hitachi Zosen Corporation

Takeda Pharmaceutical Company Limited

Takeda Pharmaceutical Company Limitjed

Hideki Saito

Tsuneo Yasuma

Toshiaki Nagata

Mitsuru Nonogawa

Toshiyuki Okada

Katsuya Sasaki

Toshiro Saeki

Motomitsu Suzuki

Masato Aoki

Shigeharu Okumura

Kyoji Chikira

Yoichiro Nakamura

In this method, an oligonucleotide represented by formula (II) [wherein Y, Q, Base, r and r' are each as defined in claim] is prepared by using, as a synthesis unit, a novel nucleoside monomer compound represented by formula (I) [wherein X, R, Y, Base, Z, Ar, R, Rand n are each as defined in claim]. The novel nucleoside monomer compound is a nucleoside, the base moiety of which is substituted with an aromatic-hydrocarbon-ring-carbonyl or -thiocarbonyl group having at least one hydrophobic group. The method can dispense with column-chromatographic purification in every reaction, and enables base elongation not only in the 3&#x2032;-direction but also in the 5&#x2032;-direction, thus attaining efficient liquid-phase mass synthesis of an oligonucleotide. 

Method for liquid-phase synthesis of nucleic acid

In this method, an oligonucleotide is prepared by using, as a synthesis unit, a novel nucleoside monomer compound represented by formula (I) [wherein X, R, Y, Base, Z, Ar, R, Rand n are each as defined in Claim]. The novel nucleoside monomer compound is a nucleoside, the base moiety of which is substituted with an aromatic-hydrocarbon-ring-carbonyl or -thiocarbonyl group having at least one hydrophobic group. The method cars dispense with column-chromatographic purification in every reaction, and enables base elongation not only in the 3'-direction but also in the 5&#x2032;-direction, thus attaining efficient liquid-phase mass synthesis of an oligonucleotide. 

A method in which a pressure in an imaginary vacuum container is determined by calculation based on a gas flow rate difference between an estimated discharge gas amount determined from the actual opening degree of a vacuum valve provided on the discharge conduit of a vacuum container and an estimated inflow gas amount determined in the preceding operation period, an estimated inflow gas amount at the present time is determined based on a difference between the calculated pressure and a pressure measured in an actual vacuum container by a pressure meter, a set discharge gas amount is determined by adding, to the estimated inflow gas amount, a correction gas amount obtained based on a pressure deviation between a set pressure and the measured pressure, and the degree of opening of the vacuum valve is controlled such that a discharge gas amount reaches the set discharge gas amount.

Method and device for controlling pressure of vacuum container

A vibrating apparatus for vibrating a mold 1 by an electrohydraulic stepping cylinder 5 through a link mechanism 3 is characterized in that when a driving signal delivered into a drive unit 26 for the stepping cylinder 5, the actual acceleration of the mold 1 is fed back to a target waveform signal for the mold 1 and compensating signals for cancelling the operation delay of the stepping cylinder 5 and the signal transfer lag due to elastic deformation of the link mechanism and the like are added thereto to make feed-forward compensation.

Mold vibrating apparatus in continuous casting equipment

Growing community of inventors

Osaka, Japan

Hideki Saito