Toray Industries, Inc.

Other

Osaka University

Tadatsugu Taniguchi

Taniguchi, Tadatsugu

Hideki Narumi

Koji Yamashita

Miyuki Sato

Yoshiaki Tsushima

Saburou Sone

Shizuo Akira

Tomokatsu Iwamura

Hajime Masumoto

Akihito Kaneda

Akiko Soneda

A novel immunostimulatory oligonucleotide by which an IFN-inducing activity is enhanced and an inflammatory cytokine-inducing activity is reduced, and a pharmaceutical containing the same, and an application thereof are provided. That is, the present invention provides the immunostimulatory oligonucleotide composed of a base sequence represented by a formula: 5'-(G)PXCGYQ(G)-3&#x2032; (SEQ ID NO: 118) (X and Y are mutually independent and represent an arbitrary sequence which has a length of 0 to 10 nucleotides and does not contain 4 or more consecutive G residues, and a length of X+Y is 6 to 20 nucleotides; XCGY contains a palindrome sequence having a length of at least 8 nucleotides and has a length of 8 to 22 nucleotides; P and Q are mutually independent and represent one nucleotide other than G; M represents an integer of 6 to 10 and N represents an integer of 0 to 3) wherein a full length thereof is 16 to 37 nucleotides (except for an oligonucleotide composed of a base sequence represented by SEQ ID NO:5), the pharmaceutical application thereof.

Immunostimulatory oligonucleotides and use thereof in pharmaceuticals

A method for producing an interferon-&#x3b2; complex includes binding interferon-&#x3b2; to polyethylene glycol in the presence of at least one additive selected from the group consisting of oligosaccharides having 5 or less sugar units, monosaccharides, sugar alcohols thereof, and Cpolyhydric alcohols.

Method of producing an interferon-&#x3b2; complex

The present invention relates to a complex between interferon-&#x3b2; and polyethylene glycol, which has high biological activity, and to a method for producing the complex at high efficiency. Namely, the present invention relates to a method for producing an interferon-&#x3b2; complex comprising binding interferon-&#x3b2; to polyethylene glycol in the presence of at least one additive selected from the group consisting of oligosaccharides having 5 or less sugar units, monosaccharides, their corresponding sugar alcohols, and Cpolyhydric alcohols, and to an interferon-&#x3b2; complex produced by the method, which has polyethylene glycol specifically bound with lysine located at the 19th or 134th position in the amino acid sequence of interferon-&#x3b2;.

Interferon-&#x3b2; complex

Growing community of inventors

Kanagawa, Japan

Hideki Narumi