Kyowa Hakko Bio Co., Ltd.

Kyowa Hakko Kogyo Co., Ltd.

Agency of Industrial Science and Technology

Kyowa Hokkoh Kogyo Co., Ltd.

Hideki Murata

Hiroshi Nagano

Tsuyoshi Shimose

Kenji Tajima

Hideo Kawabe

Ryuichiro Kurane

Shuichi Ishino

Michio Shiomi

Yasuhiro Nohata

Tsuyoshi Mokudai

Noboru Fujii

Ikuhiro Nakatani

Mariko Shitashige

Takahiro Sakuma

Tadashi Hayashi

Seiji Sugimoto

Akira Yotsuji

A method of purifying cytidine diphosphate choline, which comprises contacting a cytidine diphosphate choline solution containing a nucleic acid analogue and having a pH of not less than 0.5 and not more than 5.0 with an H-type strongly acidic cation exchange resin, and eluting cytidine diphosphate choline adsorbed onto the resin with water or an aqueous solution having an ion concentration of not more than 0.1 mol/L to separate and purify the cytidine diphosphate choline.

Method of purifying cytidine diphosphate choline

Disclosed is a crystal of glutathione having an average width of 7 to 40 &#x3bc;m and an average particle diameter of 10 to 60 &#x3bc;m, preferably having an angle of repose of 53 degree or less, more preferably having a crude specific volume of 5.0 cm/g or less, still more preferably in the form of an alpha-form crystal. Also disclosed is a tablet containing such a crystal of glutathione. Further disclosed is a process for producing such a crystal of glutathione.

Crystal of glutathione and process for production thereof

In the method for separating and purifying histidine from a culture containing the amino acid, the culture containing histidine and microbial cells is charged onto the top of a column filled with a carrier particle whose particle size is 420 &#x3bc;m or more and which has an ability to adsorb histidine and then an eluent is passed through the column whereby accomplishing the separation and purification of histidine, and preferably in the step mentioned above, a strong acid cation exchange resin is employed as a carrier particle whereby accomplishing the separation and purification of histidine.

Method for purifying histidine from a cell culture

The present invention provides crystals of L-ornithine and a process for producing the crystals of L-ornithine including the steps of (i) exposing L-ornithine or a salt thereof, a composition comprising L-ornithine or a salt thereof, or a solution containing L-ornithine, a salt thereof, or the composition to a cation exchange resin to adsorb L-ornithine onto the cation exchange resin; (ii) eluting L-ornithine from the cation exchange resin on which L-ornithine is adsorbed with an aqueous alkaline solution, and removing an alkaline component from the resulting eluate to prepare an aqueous L-ornithine solution; and (iii) mixing the aqueous L-ornithine solution with a hydrophilic organic solvent, and crystallizing L-ornithine from the resulting mixed solution.

Crystals of L-ornithine and process for producing the same

In the method for separating and purifying a basic amino acid, preferably the basic amino acid selected from arginine, lysine or ornithine from a culture containing the amino acid, the culture containing the a basic amino acid and microbial cells, preferably the culture whose pH is 4 to 10, is charged onto the top of a column filled with a weak acid cation exchange resin whose particle size is 300 &#x3bc;m or more and passed therethrough, and then an eluent is poured into the column from the top thereof whereby accomplishing the separation and the purification of the basic amino acid.

Methods for purifying amino acids

The present invention provides crystals of glutathione oxidized n hydrate (wherein n is an integer or a fraction of not less than 0 and less than 8) useful, for example, as final products of, as raw materials for, or as intermediates of health foods, pharmaceuticals, cosmetics or the like, and a process for producing crystals of glutathione oxidized, which comprises a step of crystallizing glutathione oxidized from a mixture of an aqueous solution containing glutathione oxidized and a water-miscible organic solvent and which is suitable for large-scale synthesis or industrialization.

Crystals of oxidized glutathione and process for producing the same

It is an object of the present invention to provide a method of stabilizing reduced nicotinamide adenine dinucleotide or reduced nicotinamide adenine dinucleotide phosphate [hereinafter abbreviated as NAD(P)H] and a preparation containing NAD(P)H stabilized by the method. As the method of stabilizing NAD(P)H, a method of adding astaxanthin to NAD(P)H is provided.

Method of stabilizing reduced nicotinamide adenine dinucleotide or reduced nicotinamide adenine dinucleotide phosphate

Polysaccharide and a method of producing it

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Hofu, Japan

Hideki Murata