National Institute of Advanced Industrial Science and Technology

Tokyo Medical University

Hideki Matsuzaki

Hisashi Narimatsu

Yuzuru Ikehara

Atsushi Kuno

Masayuki Noguchi

Akira Togayachi

Masaharu Nomura

Hiroyuki Kaji

Takashi Ohkura

Yoshitoshi Hirao

Jun Iwaki

Minako Abe

Jun Hirabayashi

Yasuhiro Hashimoto

Keiro Shirotani

Hiromi Ito

Satoshi Futakawa

Hajime Arai

Masakazu Miyajima

Kazuo Fujihara

An object of the present invention is to develop and provide a lung cancer differential marker with which lung cancer can be diagnosed conveniently and highly sensitively without depending only on increase or decrease in protein expression level between cancer patients and healthy persons. Another object of the present invention is to develop and provide a glycan marker capable of distinguishing histological types of lung cancer. Of serum glycoproteins, glycopeptide and glycoprotein groups whose glycan structures were altered specifically in lung cancer cell culture supernatants were identified, and they are provided as lung cancer differential markers.

Lung cancer differential marker

The purpose of the present invention is to develop: a method for selectively separating a glycoprotein derived from the central nervous system from a body fluid or a central nervous system cell; and a method for searching for an index marker for central nervous system diseases, which utilizes the aforementioned method. A protein derived from the central nervous system, which occurs in a trace amount in a body fluid or a central nervous system cell, can be selectively enriched by a two-stage separation procedure comprising removing a glycoprotein having sialic acid at a non-reducing terminal thereof from the body fluid or the central nervous system cell and then separating a glycoprotein having N-acetylglucosamine at a non-reducing terminal thereof.

Method for enrichment and separation of spinal fluid glycoprotein, method for searching for marker for central nervous system diseases which utilizes the aforementioned method, and marker for central nervous system diseases

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Tsukuba, Japan

Hideki Matsuzaki