Nitto Boseki Co., Ltd.

Nippon Telegraph and Telephone Corporation

Ntt Advanced Technology Corporation

Kao Corporation

Hideki Ishihara

Natsuki Sato

Masayuki Wakabayashi

Ryo Nagase

Hiroshi Tanaka

Shinichi Iwano

Hiroyuki Terazaki

Fumihiro Ashiya

Mitsuo Kama

Hiroshi Aoyama

Yasuo Oda

Mika Kuroiwa

Masatoshi Nakatsuji

Yoshihiro Takada

Hirohisa Fujihara

Provided are a pretreatment method for detecting the number of cells containing Ki-67 protein-positive nuclei using Ki-67 antibody; a method for the detection; a kit to be used in the detection method; and determination of a therapy regimen using the aforesaid method. Attempts were made to activate Ki-67 antigen with the use of an enzyme having been considered as inappropriate for the activation thereof. By pretreating a sample with an enzyme not recognizing the epitope of MIB-1 (a rare cutter enzyme), the antigen activation of Ki-67 antigen was enhanced, while enhancing the antigenicity of other antigens including a cytokeratin too. As a result, a method for more objectively and more universally quantifying Ki-67-positive cells at higher reproducibility, said method comprising isolating cell nuclei from an FFPE section while enhancing the antigenicity and performing a reaction between Ki-67 protein, i.e., the target, existing in the cell nuclei with a fluorescently labeled antibody, has been completed.

Method for isolating cell nuclei having enhanced antigenicity from fixed cells or FFPE tissue section, and antigen activator and kit therefor

The present invention addresses the problem of providing a novel method for classifying cancer cells by an analysis method in which measurement of the activity of two types of protein kinase is used. Cancer cells are newly classified and drug sensitivity is predicted on the basis of the ratio of the activity of two types of protein kinase derived from the same sample.

Method for determining drug-sensitive human cell lines by analysis method in which measurement of activity of two types of protein kinase is used

An optical connector comprising: first and second movable membersheld on an optical connector bodyto be axially movable in a longitudinal direction; locking means for locking the first and second movable members when the first movable member is located at a predetermined locking position in a tip part of the optical connector body, and integrally moving the first and second movable members; and unlocking means formed in the optical connector body, and including a locking acting portion which acts on the locking means when the first movable member is located rearwardly of a predetermined unlocking position in a rear part of the optical connector body, to unlock the first and second movable members, and which locks the first and second movable members when the first movable member is located forwardly of the unlocking position, and a second movable member hooking portionfor inhibiting rearward movement of the second movable member after the first and second movable members are unlocked, and wherein the second movable member has a functional portion having a predetermined function, and when the second movable member is located at a predetermined position in the optical connector body, the function acts on a ferruleor an optical fiberto fulfill the function.

Optical connector

Growing community of inventors

Tokyo, Japan

Hideki Ishihara