Suntory Limited

Hideki Adachi

Hiroyuki Arai

Keizo Inoue

Masafumi Tsujimoto

The present invention relates to an isolated human protein having platelet activating factor acetylhydrolase activity.

Platelet activating factor acetylhydrolase, and gene thereof

An oxidized phospholipid degrading enzyme is provided, which plays an important role in the oxygen stress preventive mechanism in organisms. The enzyme hydrolyzes a 1-acyl-2-.omega.-carboxyfatty acid acyl-3-phosphatidylcholine as a substrate at the 2-ester bond thereof to form 1-acyl-2-lyso-3-phosphatidylcholine. The activity of the enzyme is slightly enhanced by 4 mM calcium chloride. The molecular mass of the enzyme is 95.+-.5 kDa (by gel filtration). The enzyme is composed of three subunits whose molecular masses have been found to be 29 kDa, 30 kDa and 45 kDa, respectively, by SDS-polyacrylamide electrophoresis. A gene coding the enzyme is also provided. This gene is important for the synthesis of the enzyme by genetic engineering.

Oxidized phospholipid degrading enzyme and gene thereof

This invention relates to an isolated and purified DNA encoding an amino acid sequence which comprises SEQ. ID. NO:3.

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Asaka, Japan

Hideki Adachi